|Table of Contents|

Effects of HS3ST1 on proliferation，migration，invasion and cisplatin resistance of lung cancer cell

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 03
Page:
365-369
Research Field:
Publishing date:

Info

Title:
Effects of HS3ST1 on proliferation，migration，invasion and cisplatin resistance of lung cancer cell
Author(s):
LI Meifang1; LIN Jinghui1; LIAO Jinrong2; HE Zhiyong1
1.Department of Thoracic Medical Oncology;2.Department of Radiobiology，Fujian Cancer Hospital & Fujian Medical University Cancer Hospital，Fujian Fuzhou 350014，China.
Keywords:
lung cancer; proliferation; migration; invasion; cisplatin; drug resistance
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2021.03.001
Abstract:
Objective：To study the effects of HS3ST1 on proliferation，migration,invasion and cisplatin resistance of lung cancer cell line.Methods：HS3ST1 overexpression cell line was constructed by lentivirus infection.The expression of HS3ST1 was detected by RT-PCT and Western blot.The effects of HS3ST1 overexpression on proliferation，migration,invasion and cisplatin resistance of A549 cells were detected by CCK8 test，Transwell test，Invsion test and cytotoxicity test.Proteins in NF-κB pathway were evaluated by Western blot.The GEO database (GSE108214) was used for cisplatin resistance analysis，and the GEO database (GSE30219) data was used to analyze the effect of HS3ST1 expression on the survival time of patients with lung cancer.Results：The expression of HS3ST1 was higher in lung cancer cells and lung cancer tissues，compared to normal lung cells and non-cancer lung tissues.The expression of HS3ST1 in middle and late stage (Ⅲ-Ⅳ stage) was higher than that in early stage (Ⅰ-Ⅱ stage).Overexpression of HS3ST1 promoted the proliferation,migration and invasion of A549 cells.GEO database (GSE108214) analysis showed that cisplatin resistant cell lines highly expressed HS3ST1.Cytotoxicity assay showed that overexpression of HS3ST1 led to cisplatin resistance in lung cancer cells.GEO database (GSE30219) data showed that the total survival time of lung cancer patients with high expression of HS3ST1 was significantly shortened.Conclusion：HS3ST1 promoted the proliferation，migration,invasion and cisplatin resistance of lung cancer cells by activating NF-κB signaling pathway，and the prognosis of lung cancer patients with higher expression of HS3ST1 is significantly poor than those with lower expression of HS3ST1.

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福建省卫生计生青年科研课题（编号：2017-2-8）
Last Update: 2020-12-31