|Table of Contents|

Study of bakuchiol induced apoptosis through MAPK pathways in human hepatocellular carcinoma HepG2 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 05
Page:
729-735
Research Field:
Publishing date:

Info

Title:
Study of bakuchiol induced apoptosis through MAPK pathways in human hepatocellular carcinoma HepG2 cells
Author(s):
Long Xue12Qiu Wanting3Cao Shijie1Ding Liqin1
1.Tianjin Key Laboratory of Modern Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;2.Department of Biochemistry and Molecular Biology,Faculty of Life Sciences and Biological Pharmacy,Shenyang Pharmaceutical University,Liaoning Shenyang 110016,China;3.Nankai High School,Tianjin 300100,China.
Keywords:
bakuchiolMAPKHepG2 cellsapoptosis
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2020.05.008
Abstract:
Objective:To investigate the antitumor effect and molecular mechanism of bakuchiol against hepatocellular carcinoma cells.Methods:The growth inhibitory effect of bakuchiol on HepG2 cells was tested by MTT assay.Fluorescence microscope and inverted microscope were used to observe cell apoptosis.In addition,Western blotting detected the expression of apoptosis related proteins and MAPK family.Furthermore,the inhibitors of MAPK family protein were used to investigate the inhibitory effect and the levels of apoptosis related proteins.Results:Bakuchiol inhibited the proliferation of HepG2 cells in a dose-dependent manner,which showed stronger growth inhibitory effect than 5-fluorouracil by MTT assay.Meanwhile,bakuchiol showed low cytotoxicity in normal human liver L02 cells.Furthermore,bakuchiol could induce apoptosis in HepG2 cells.In addition,MAPK family was involved in bakuchiol-caused apoptosis.Bakuchiol dose-dependently activated the JNK MAPK,which promoted apoptosis.The activation of survival ERK was suppressed by bakuchiol,while the expression of p38 had no obvious change.Conclusion:The mechanism of bakuchiol-induced HepG2 cells growth inhibition was first elucidated in order to provide the basis for clinical application.

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