|Table of Contents|

Effects of safflower polysaccharide on proliferation and invasion of gastric cancer MGC-803 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 04
Page:
569-573
Research Field:
Publishing date:

Info

Title:
Effects of safflower polysaccharide on proliferation and invasion of gastric cancer MGC-803 cells
Author(s):
Liu Chao; Ji Le; Bai Tiecheng
Department of Gastrointestinal Surgery,Affiliated Hospital of Yan'an University,Shaanxi Yan'an 716000,China.
Keywords:
gastric cancer; safflower polysaccharide; cell proliferation; cell cycle; invasion; Wnt/β-catenin signaling pathway
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2020.04.010
Abstract:
Objective：To investigate the effects of safflower polysaccharide on the proliferation and invasion of gastric cancer MGC-803 cells and its molecular mechanism.Methods:MGC-803 cells were treated with safflower polysaccharides at different concentrations (0,0.16,0.32 and 0.64 mg/ml) for 48 hours.The proliferation,cloning,cell cycle distribution and invasiveness of MGC-803 cells were detected by MTT,cloning assay,flow cytometry and Transwell method respectively.The expression of proteins related to Wnt/β-catenin signaling pathway,such as β-catenin,c-Myc,CyclinD1,VEGF and MMP-9,were detected by Western blot.MGC-803 cells were treated with 0.32 mg/ml safflower polysaccharide and Wnt/β-catenin signaling pathway inhibitor VXAV939 alone or in combination for 48 hours,the proliferation and invasion of MGC-803 cells were further observed.Results:Compared with the control group (0 mg/ml),the cell proliferation rate,clone formation rate,number of invasive cells and the expression level of intracellular β-catenin,c-Myc,CyclinD1,VEGF and MMP-9 proteins in the 0.16,0.32 and 0.64 mg/ml safflower polysaccharides treatment groups were significantly decreased,while the percentage of cells in G0/G1 phase was significantly increased (P＜0.05).After using VXAV939,the above effects of safflower polysaccharide on MGC-803 cells were further enhanced.Conclusion:Safflower polysaccharide can inhibit the proliferation and invasion of gastric cancer MGC-803 cells and induce cell cycle arrest at G0/G1 phase.Its mechanism may be related to the inhibition of Wnt/β-catenin signaling pathway.

References:

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延安市科学技术研究发展计划项目（编号：2013-kw06）
Last Update: 2020-01-14