|Table of Contents|

The influence of microRNA-192 on proliferation，migration and invasion of RKO colon cancer cells and mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 03
Page:
374-379
Research Field:
Publishing date:

Info

Title:
The influence of microRNA-192 on proliferation，migration and invasion of RKO colon cancer cells and mechanism
Author(s):
Fan Panhong1; Zhao Wenyue2; Jia Linjiao3; Liu Qiuyu1; Zhao Yuewu1
1.Pathology Department;3Breast Surgery,Henan Provincial People's Hospital，Henan Zhengzhou 450003，China；2.Endocrinology Department,Dandong Central Hospital，Liaoning Dandong 118010，China.
Keywords:
microRNA-192; RKO colon cancer cells; E-cad; VEGF
PACS:
R735.3＋5
DOI:
10.3969/j.issn.1672-4992.2020.03.005
Abstract:
Objective：To investigate the influence of microRNA-192 (miR-192) on proliferation，migration and invasion of RKO colon cancer cell and the potential mechanism.Methods：miR-192 mimics (miR-192) was transfected into human colon cancer cell line RKO using Oligofectamine.qRT-PCR was used to measure the expression level of miR-192.The proliferation of miR-192 was measured by CCK-8 and colony formation assays.Wound-healing，Transwell chamber were used to evaluate the migration and invasion ability.Western blot detected the effect of miR-192 on E-cad and VEGF expression.Results：The CCK-8 and colony formation assays showed that miR-192 can inhibit the proliferation of RKO cells.The number of migrating cells reduced sharply by cell counting of Wound-healing and Transwell assays.The invasion of RKO cells was inhibited compared with the Control group and NC group（P<0.05）.Western blot assay revealed that E-cad increased and VEGF decreased significantly by miR-192.Conclusion：miR-192 could effectively inhibit the growth and invasion and migration ability of colon cancer cells.miR-192 increased E-cad and decreased VEGF expression may play an important role in the process.The findings of this study suggest that miR-192 may have a unique potential as a novel biomarker candidate for tumor diagnosis and therapy.

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河南省医学科技攻关计划项目（编号：201602248）
Last Update: 1900-01-01