|Table of Contents|

Enhancement of radiation effects by Osthole on human gastric BGC-823 cell line in vitro

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 03
Page:
379-382
Research Field:
Publishing date:

Info

Title:
Enhancement of radiation effects by Osthole on human gastric BGC-823 cell line in vitro
Author(s):
Mao JialeiYang HuaLu QiongJin Lijuan
Department of Oncology,Kunshan Hospital of Traditional Chinese Medicine,Jiangsu Kunshan 215300,China.
Keywords:
Ostholecell clone formation assaycell cyclecell apoptosis
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2020.03.006
Abstract:
Objective:To study the enhancement of radiation effects by Osthole on gastric BGC-823 cells in vitro.Methods:MTT assay detected the effects of single drug and combined radiotherapy on BGC-823 cells.Cell clone formation assay detected the enhancement of radiation effects by Ost on BGC-823 cells.Flow cytometry analyzed the effects of Ost and radiotherapy on cell cycle and apoptosis in BGC-823 cells.Results:Ost could inhibit the proliferation of BGC-823 cells.Low cytotoxic dose of Ost (15 μg/ml) had radiotherapy sensitization effect on BGC-823 cells.After single-click multi-target model fitting,the cell surviving fraction of Ost combined radiotherapy group was shifted to the left,compared with the radiotherapy group,and D0 and Dq values were reduced,and the radiotherapy sensitization ratio SER=1.64.Conclusion:Low cytotoxic dose of Ost (15 μg/ml) has radiotherapy sensitization effect on BGC-823 cells.The mechanism may be related to increasing radiation-induced apoptosis,causing cell block in phase G1 when there is radiotherapy sensitization,and reducing the proportion of cells in phase S when there is radiotherapy resistance.

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