|Table of Contents|

miR-124-3p inhibits cell proliferation and invasion in cervical squamous cell carcinoma by targeting FOXQ1

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 02
Page:
272-278
Research Field:
Publishing date:

Info

Title:
miR-124-3p inhibits cell proliferation and invasion in cervical squamous cell carcinoma by targeting FOXQ1
Author(s):
Wu Wei; Yuan Ping
Department of Obstetrics and Gynecology,Caidian District People's Hospital of Wuhan City,Hubei Wuhan 430100,China.
Keywords:
cervical squamous cell carcinoma; miR-124-3p; FOXQ1; epithelial-mesenchymal transition; proliferation; invasion
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2020.02.022
Abstract:
Objective:To investigate the expression level of miR-124-3p in cervical squamous cell carcinoma (CSCC) and its effect on cell proliferation and invasion,and to preliminarily clarify the mechanism of miR-124-3p targeting the forkhead box Q1 (FOXQ1).Methods:A total of 61 CSCC specimens were collected from January 2015 to December 2017.Expressions of miR-124-3p and FOXQ1 protein in cancer tissues and paired adjacent tissues were detected by RT-qPCR and IHC,respectively,and the correlation between miR-124-3p and FOXQ1 mRNA expression was analyzed.RT-qPCR was used to detect the expression of miR-124-3p and FOXQ1 mRNA in human CSCC lines (SiHa and CaSki) and human immortalized squamous cell lines (Ect1/E6E7).CaSki cells were transfected by miR-124-3p mimics,and the effects of miR-124-3p on cell proliferation and invasion were detected by CCK-8 and Transwell assays,respectively.Western blot was used to detect the effect of miR-124-3p on FOXQ1,E-cadherin,and Vimentin protein expression levels.Finally,a dual luciferase reporter gene was used to verify the targeted regulation of miR-124-3p on FOXQ1.Results:The expression of miR-124-3p in CSCC tissues was lower than that in paired adjacent tissues (P＜0.05) and its expression in human CSCC cell lines (SiHa and CaSki) were also lower than those in human cervical immortalized squamous cell lines (Ect1/E6E7) (P＜0.05).Compared with transfected negative control cells,the proliferation and invasion of CaSki cells transfected with miR-124-3p mimics was significantly inhibited (P＜0.05).The expression of FOXQ1 mRNA and protein in CSCC tissues was significantly higher than that in adjacent tissues (P＜0.05),and further correlation analysis showed that the expression level of FOXQ1 mRNA was negatively correlated with miR-124-3p (r=-0.882,P＜0.05).After transfection of miR-124-3p mimics,the expression of FOXQ1 and Vimentin protein in CaSki cells was decreased while the expression of E-cadherin protein was increased.Dual luciferase reporter assays confirmed that miR-124-3p can target FOXQ1 expression through direct binding to the 3' UTR of FOXQ1 mRNA.Conclusion:miR-124-3p is down-regulated in CSCC,which influences the epithelial-mesenchymal transition state of cells through targeted regulation of FOXQ1 expression,thereby affecting the proliferation and invasion of CSCC cells.

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Last Update: 2019-11-29