|Table of Contents|

The effect of miR-19b on the invasion and migration in ovarian cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 01
Page:
9-12
Research Field:
Publishing date:

Info

Title:
The effect of miR-19b on the invasion and migration in ovarian cancer cells
Author(s):
Li Yanan; Du Xiaobing; Lu Fenghua; Zhang Suqin; Xie Ya
Department of Obstetrics and Gynecology，the First Affiliated Hospital of Zhengzhou University，Henan Zhengzhou 450052，China.
Keywords:
ovarian cancer; microRNA-19b; PTEN/AKT; invasion; migration
PACS:
R737.31
DOI:
10.3969/j.issn.1672-4992.2020.01.003
Abstract:
Objective：To investigate the mechanism of microRNA-19b(miR-19b) in the migration and invasion of ovarian cancer.Methods：qRT-PCR was used to detect the expression of miR-19b in human normal ovarian tissue，ovarian cancer tissue and ovarian cancer cell lines(SKOV-3 and OVCAR-3).miR-19b inhibitor or the negative control was transfected into OVCAR-3 cells.Transwell assay was performed to detect the effect of miR-19b on the migration and invasion of ovarian cancer cells.Western blot was used to analyze the effects of miR-19b on the expression of PTEN and other relevant proteins of PTEN/AKT signaling pathway.Dual reporter gene assay was used to detect the interaction between miR-19b and the 3’-UTR of PTEN.Results：The relative expression of miR-19b was significantly higher in ovarian cancer tissue(3.56±0.68，P<0.05) and ovarian cancer cell lines SKOV-3(3.01±0.02，P<0.05) and OVCAR-3(3.78±0.04，P<0.05) than that in normal ovarian tissue.Transwell assay showed that abilities of migration and invasion in OVCAR-3 cells were inhibited when miR-19b was down-regulated.Meanwhile，overexpression of miR-19b significantly reduced the expression of PTEN in OVCAR-3 cells and increased the phosphorylation level of AKT protein.Dual reporter gene assay revealed that miR-19b may interact the 3’-UTR of PTEN to reduce the firefly luciferase activity of the recombinant vector.Conclusion：miR-19b is highly expressed in ovarian cancer tissue and cell lines，which may promote the migration and invasion of ovarian cancer cells，and may be a potential biomarker and therapeutic target for ovarian cancer patients.

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Memo

Memo:
National Natural Science Foundation of China(No.81202070）；国家自然科学基金青年基金（编号：81202070）;河南省高等学校重点科研项目(编号:18A320079,19A320089）
Last Update: 1900-01-01