|Table of Contents|

Research of EBSS starvation induced autophagy and its mechanism in CNE-2 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 01
Page:
30-35
Research Field:
Publishing date:

Info

Title:
Research of EBSS starvation induced autophagy and its mechanism in CNE-2 cells
Author(s):
Liu Ruiyin; Wu Mengge; Zeng Wen; Zhang Rui; Li Shiqin; Fei Jimin
Department of Head and Neck，Third Affiliated Hospital of Kunming Medical，Yunnan Kunming 650118，China.
Keywords:
nasopharyngeal neoplasms; autophagy; hunger; LC3; LKB1; Beclin 1
PACS:
R739.6
DOI:
10.3969/j.issn.1672-4992.2019.01.008
Abstract:
Objective：To investigate starvation induced autophagy and mechanism in normal nasopharyngeal cell line NP69 and nasopharyngeal carcinoma CNE-2 cell，to provide evidence for the application of Baf-A1 in nasopharyngeal carcinoma.Methods：NP69 and CNE-2 cell were starvation induced by EBSS medium instead of DMEM medium，Baf-A1 inhibit autophagy of CNE-2 cell.Western blot were used to detect the special markers of autophagic microtubule-associated protein 1 light chain 3(LC3)，and transmission electron microscope(TEM) was used to observe the autophagic body.In condition，cell proliferation and apoptosis induced by nutrient depletion was measured by MTT assay.qRT-PCR analysis of the mRNA expression of autophagy related genes.Results：Compared with NP69，CNE-2 cell autophagy activity was significantly promoted in stavation condition induced by EBSS solution.The cell apoptosis was delayed by 100 nmol/L Baf-A1 which inhibit autophagy of CNE-2 cell.Meanwhile，the results of qRT-PCR demonstrated EBSS significantly promoted mRNA levels of LKB1，AMPK，ULK1，Beclin1，ATG5 in CNE-2 cells(P<0.05).Conclusion：The starvation condition may induce autophagy of CNE-2 cells by LKB1/AMPK/mTOR pathway.

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Memo

Memo:
云南省卫生高层次人才基金资助项目(编号：D-201659)
Last Update: 2018-11-30