|Table of Contents|

The effect of miR-34a on proliferation and apoptosis of endometrial cancer cells by targeting Notch1

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 01
Page:
26-29
Research Field:
Publishing date:

Info

Title:
The effect of miR-34a on proliferation and apoptosis of endometrial cancer cells by targeting Notch1
Author(s):
Li Yanwei1Liu Yiwei2
1.Department of Gynaecology;2.Department of Pharmacy,Laiwu City People's Hospital,Shandong Laiwu 271100,China.
Keywords:
endometrial cancermiR-34aapoptosisNotch
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2019.01.007
Abstract:
Objective:To investigate the effect of miR-34a on proliferation and apoptosis of endometrial cancer by targeting Notch1.Methods:The expression of miR-34a in endometrial cancer and paracancerous tissues was detected by fluorogenic quantitative PCR.The downstream target gene of miR-34a was detected by dual luciferase reporter gene assay.Ishikawa cells were transferred with miR-NC,miR-34a mimics and miR-34a mimics+Notch1.The cell viability was detected by MTT assay and the cell apoptosis was detected by flow cytometry.The expression of Notch1 protein was detected by Western blot.Results:The expression of miR-34a in endometrial cancer tissues was lower than that in paracancerous tissues (P<0.05).Dual-luciferase reporter assay confirmed that miR-34a could bind to Notch1 3' UTR.miR-34a mimics significantly inhibited cell viability (P<0.05) and promoted apoptosis (P<0.05),compared with miR-NC.miR-34a mimics significantly inhibited Notch1 protein expression (P<0.05).Conclusion:miR-34a can inhibit the proliferation of endometrial cancer cells and promote apoptosis by regulating the expression of target gene Notch1.

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Memo:
莱芜市科学技术项目(编号:2016078)
Last Update: 2018-11-30