|Table of Contents|

Experimental study on the function of CpG combined with MUC1 in enhancing dendritic cell function in human peripheral blood

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 04
Page:
525-528
Research Field:
Publishing date:

Info

Title:
Experimental study on the function of CpG combined with MUC1 in enhancing dendritic cell function in human peripheral blood
Author(s):
Xu Fu1Xie Yinghong1Yang Xiangyun1Liu Yun1Zheng Xiaohua1Wu Yan1Zhang Hao2Xu Lu2
1.Cadre's Ward;2.Clinical Laboratory,202 Hospital of the PLA,Liaoning Shenyang 110003,China.
Keywords:
CpGmucin 1 (MUC1)dendritic cell (DC)
PACS:
R73-35.1
DOI:
10.3969/j.issn.1672-4992.2017.04.005
Abstract:
Objective:To observe the effect of CpG combined with mucin 1 (MUC1) on the proliferation of dendritic cells (DC) stimulated T cells.Methods:Lymphocyte separation liquid was used to isolate from human peripheral blood mononuclear cells and cultured in vitro cell cytokines GM-CSF,IL-4 and TNF-α induced DC.Cell morphology was observed by flow cytometry cell markers CD80 and CD86.Harvest DC according to the following groups was divided into A:DC,B:DC+CpG,C:DC+MUC1,D:DC+CpG+MUC1,E:CpG+MUC1,respectively.T lymphocyte was in mixed culture,the proliferation of T cells by MTT assay.Results:DC phenotype detection results:CD80+ cells accounted for 70.4%,CD86+ cells accounted for 72.0%,showing a mature DC phenotype.MUC1 and DC combined vaccine and mixed lymphocyte culture showed that DC had the role of T cell proliferation stimulation.CpG+DC group and MUC1+DC group,respectively,compared with pure DC group,T cell proliferation was significantly enhanced (P<0.01).CpG+MUC1+DC was higher than that with MUC1 or CpG+DC group,differences were significant (P<0.01),the simple addition of CpG and MUC1 (DC-free group) without significantly stimulating the proliferation of T cells.Conclusion:CpG combined with MUC1 can significantly improve the function of DC in promoting the proliferation of T cells.

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Memo

Memo:
辽宁省自然科学基金资助项目(编号:2013020181)
Last Update: 2016-12-29