|Table of Contents|

miR103 reversed the resistance of cisplatin in prostatic cancer cells by targeting PDCD10

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 04
Page:
511-515
Research Field:
Publishing date:

Info

Title:
miR103 reversed the resistance of cisplatin in prostatic cancer cells by targeting PDCD10
Author(s):
Fu Xiaoliang; Yang Zengyue; Shu Tao; Wang Dong
Department of Urology，Tangdu Hospital Affiliated to the Fourth Military Medical University,Shaanxi Xi'an 710038,China.
Keywords:
miR103; PDCD10; P-gp; prostatic cancer
PACS:
R737.25
DOI:
10.3969/j.issn.1672-4992.2017.04.002
Abstract:
Objective:To explore the effect of miR103 on the resistance of cisplatin in prostatic cancer cells and its underlying mechanism.Methods:The expression of miR103 in human prostatic cancer PC-3,LNCap,22Rv1,DU145 cell lines and cisplatin-resistant PC-3/DDP,LNCap/DDP,22Rv1/DDP,DU145/DDP cell lines were determined by RT-PCR.The PC-3/DDP cell line was chosen as experimental object.miR103 mimic or inhibitor was transfected into the cells,and cell apoptosis was detected by flow cytometer.The miR103 mimic or PDCD10 overexpression vector was transfected into the cells respectively and the expression levels of PDCD10 were determined by Western blotting and RT-PCR.PDCD10 overexpression vectors were transfected into miR103 mimic transfected cells,and cell apoptosis was detected by flow cytometer.The expression of P-gp was determined in miR103 overexpressing and PDCD10 overexpressing cells by Western blotting and RT-PCR.Results:The expression level of miR103 was significantly decreased in cisplatin-resistant cell lines compared with parent cell lines.Cell apoptosis rate was significantly increased in miR103 mimic transfected PC-3/DDP cells,but decreased slightly in miR103 inhibitor transfected cells.The expression of PDCD10 was significantly decreased in miR103 mimic transfected PC-3/DDP cells.Overexpression of PDCD10 significantly reduced cell apoptosis in miR103 mimic transfected PC-3/DDP cell,and neutralized the inhibition of P-gp expression induced by miR103 mimic.Conclusion:miR103 can reverse drug resistance of prostatic cancer cells though inhibiting the expression of P-gp by targeting PDCD10.

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Memo

Memo:
中国人民解放军第四军医大学校基金（编号：2016XD284）
Last Update: 2016-12-29