|Table of Contents|

Screening of potential proteins interacting with histone deacetylase 1

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 04
Page:
516-520
Research Field:
Publishing date:

Info

Title:
Screening of potential proteins interacting with histone deacetylase 1
Author(s):
Chen Xia1; Zeng Yan2; Zhang Yi2; Pang Xueli3; Zhang Jiefeng2
1.Department of Pediatrics，Affiliated Hospital of Chengdu University,Sichuan Chengdu 610081,China;2.Department of Hepatobiliary Surgery,Chongqing Cancer Institute,Chongqing 400030,China;3.Department of Oncology,Southwest Hospital,Third Military Medical University,Chongqing 400038，China.
Keywords:
histone deacetylase 1; esophageal cancer cell; colon cancer cell; mass spectrum
PACS:
R735.1；R735.35
DOI:
10.3969/j.issn.1672-4992.2017.04.003
Abstract:
Objective:To screen potential proteins interacting with histone deacetylase 1 (HDAC1).Methods:Expression plasmid of Flag-tagged HDAC1 was constructed and transfected into esophageal cell line EC109 and colon cancer cell line HCT116.After transfection,cells were harvested and protein expression of HDAC1 was detected by Western blotting.Protein samples of immunoprecipitation by using Flag antibody were analyzed by mass spectrum.Results:Flag-tagged HDAC1 was successfully cloned into expression vector by RT-PCR.Forty-eight hours after transfection,Flag-tagged HDAC1 was efficiently expressed in both cell lines.Sufficient protein samples were obtained by immunoprecipitation with Flag antibody.After measured by mass spectrum and database retrieving,17 and 107 potential proteins interacting with HDAC1 were obtained from EC109 and HCT116 cells,respectively.Conclusion:Potential proteins interacting with HDAC1 were successfully selected from two cancer cell lines,which contributed to further analysis of the gene functions of HDAC1 and its biological significance in cancer cells.

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Last Update: 2016-12-29