|Table of Contents|

IL-24 gene affects the expression of JNK and caspase-3 on C6 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2016 06
Page:
871-874
Research Field:
Publishing date:

Info

Title:
IL-24 gene affects the expression of JNK and caspase-3 on C6 cells
Author(s):
Li Minghui1; Zhao Jingya2; Yan Yunli3; Feng Yunzhang1
1.Second Department of General Surgery;2.Department of Pathology,Handan Central Hospital,Hebei Handan 056001,China;3.Department of Cell Biology,Hebei Medical University,Hebei Shijiazhuang 050017,China.
Keywords:
IL-24; C6 glioma cell; JNK; caspase-3; cell proliferation; cell apoptosis
PACS:
R73-3；R730.264
DOI:
10.3969/j.issn.1672-4992.2016.06.006
Abstract:
Objective:We observed the effects of the inhibition effect of exogenous IL-24 gene on C6 glioma cells by studying IL-24 gene transfected C6/IL-24 cell,empty vector C6/pLXSN cell and the parent C6 cell.Methods:With the application of MTT,we observed the effects of the inhibition effect of exogenous IL-24 gene on C6 glioma cells in vitro.With the Western blot,we examined the expression of JNK and caspase-3 proteins.Results：Compared with the C6/pLXSN and parental C6 cells,the absorbance of C6/IL-24 cells was decreased,suggesting the proliferative ability of C6/IL-24 cells was attenuated.The JNK and caspase-3 expression of C6/IL-24 cells were up-regulated (P＜0.05),there was no significant difference between C6/LXSN and C6 cells(P＞0.05).Conclusion:Exogenous IL-24 gene can affect the biological characteristic of the C6 glioma cells,suppresses its proliferation.Exogenous IL-24 gene induced C6 cell apoptosis by up-regulating and activating JNK and caspase-3.

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Last Update: 2016-01-29