|Table of Contents|

Identification of adhesion molecules existence in the human high and low metastatic large cell lung cancer cell line

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2015 06
Page:
743-746
Research Field:
Publishing date:

Info

Title:
Identification of adhesion molecules existence in the human high and low metastatic large cell lung cancer cell line
Author(s):
Zheng Haixia1; Shen Donglan2; Peng An2; He Yanling2
1.Department of Health Examination;2.Department of Oncology，Peking University Shenzhen Hospital,Guangdong Shenzhen 518036，China.
Keywords:
lung cancer cell; E-cadherin; integrins; neoplasm metastasis
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2015.06.04
Abstract:
Objective:To identify the adhesion molecules existence in the human high and low metastatic large cell lung cancer cell line L9981 and NL9980.Methods:Semi-RT-PCR was used to detect the mRNA expression of E-cadherin，integrinβ1 and integrinβ3 between the human high and low metastatic large cell lung cancer cell line;flow cytometry was used to detect the different protein expression of E-cadherin，integrinβ1 and integrinβ3 between the human high and low metastatic large cell lung cancer cell line.Results:The expression of mRNA and protein of E-cadherin of the high metastatic large cell lung cancer cell line was weaker than that of the low metastatic large cell lung cancer cell line.The expression of mRNA and protein of integrinβ1 and integrinβ3 of the high metastatic large cell lung cancer cell line was stronger than that of the low metastatic large cell lung cancer cell line.Conclusion:The high expression of E-cadherin is related to low metastatic lung cancer,the high expression of integrins is related to high metastatic lung cancer.

References:

［1］ Liotta LA,Stetler-Stevenson WG.Tumor invasion and metastasis:An imbalance of positive and negative regulation［J］.Cancer Res,2009,51(1):5054-5059.
［2］Juliano RL,Varner JA.Adherion molecules in cancer:The role of integrins［J］.Curr Opin Cell Biol,2010,5(1):812-818.
［3］ 周清华，王艳萍，车国卫，等.人大细胞肺癌细胞NL9980 和 L9981的建立及其生物学特性研究［J］.中国肺癌杂志，2003,6（6）：464-468.
［4］ Kafka A,Tomas D,Bero V3,et al.Brain metastases from lung cancer show increased expression of DVL1,DVL3 and beta-catenin and down-regulation of E-cadherin［J］.Int J Mol Sci,2014,15(6):10635-10651.
［5］ Yang YL,Chen MW,Xian L.Prognostic and clinic opathological significance of downregulated E-cadherin expression in patients with non-small cell lung cancer(NSCLC):A Meta-analysis［J］.PLoS One,2014,9(6):e99763-99765.
［6］Gui G,Puddefoot JR,Vinson GP,et al.Altered cell-matrix contact;a prerequesite for breast cancer metastasis［J］.Br J Cancer,2007,75(1):623-626.
［7］ Wang D,Müller S,Amin AR,et al.The pivotal role of integrin β1 in metastasis of head and neck squamous cell carcinoma［J］.Clin Cancer Res,2012,18(17):4589-4599.
［8］Zhang PF,Zeng GQ,Yi LZ,et al.Identification of integrin β1 as a prognostic biomarker for human lung adenocarcinoma using 2D-LC-MS/MS combined with iTRAQ technology［J］.Oncol Rep,2013,30(1):341-349.
［9］Max R,Gerritsen R,Nooi Jen P,et al.Immunohistochemical analysis of integrin expression on tumor-associated ressels of human carcinomas［J］.Int J cancer,2008,71(1):320-324.
［10］ Berghoff AS,Kovanda AK,Melchardt T,et al.αⅤβ3,αⅤβ5 and αⅤβ6 integrins in brain metastases of lung cancer［J］.Clin Exp Metastasis,2014,31(7):841-851.
［11］ Tome Y,Kimura H,Maehara H,et al.High lung-metastatic variant of human osteosarcoma cells,selected by passage of lung metastasis in nude mice,is associated with increased expression of α(Ⅴ)β(3) integrin［J］.Anticancer Res,2013,33(9):3623-3627.

Memo

Memo:
国家自然科学基金资助项目（编号:30070333）
Last Update: 2015-02-02