|Table of Contents|

Mechanism of miR-130b regulating macrophage polarization

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2015 06
Page:
738-742
Research Field:
Publishing date:

Info

Title:
Mechanism of miR-130b regulating macrophage polarization
Author(s):
Zhang Liang1; Zhao Ying2; Zhang Tianbiao3
1.Department of Thoracic Surgery，Liaoning Cancer Hospital & Institute，Liaoning Shenyang 110042，China；2.Department of General Surgery，Shengjing Hospital of China Medical University，Liaoning Shenyang 110004，China；3.Department of Biochemical and Molecular Biology，China Medical University，Liaoning Shenyang 110001，China.
Keywords:
miR-130b; M1 macrophage; M2 macrophage; polarization
PACS:
R730
DOI:
10.3969/j.issn.1672-4992.2015.06.03
Abstract:
Objective:To study the mechanism of miR-130b regulating macrophage polarization.Methods:Human leukemia mononuclear THP 1 cells was induced to Mφ macrophages by PMA,then add LPS induce to M1 or add IL-4 to for M2 macrophages.The specific markers were measured using flow cytometry,Real-time PCR and Western blot methods.Secrete factors of macrophages was detected by ELISA method.Results:Compared with Mφ macrophages,CD86 high expression,TNF alpha and IL-1 beta expression and secretion levels were increased when macrophages induced by LPS,display M1 macrophage characteristics.In macrophages induced by IL-4,the positive expression of CD206 rate increased,CCL22,CCL17 expression and secretion levels were increased.MiR-130b was expressed at a higher level in M1 (P＜0.05).Overexpression of miR-130b in M2 cells decreased CCL22 mRNA and protein level,increased IL-1 β mRNA and protein level,reduced the protein expression of PPAR-gamma.Conclusion:MiR-130b regulates macrophage polarization by targeting PPAR-γ.

References:

［1］Lawrence T,Natoli G.Transcriptional regulation of macrophage polarization:enabling diversity with identity［J］.Nature Rev Immuno,2011,11 (11):750-761.
［2］Ricote M,Li AC,Willson TM,et al.The peroxisome proliferator-activated receptor-gamma is a negative regulator of macrophage activation［J］.Nature,1998,391(6662):79-82.
［3］Murray PJ,Wynn TA.Obstacles and opportunities for understanding macrophage polarization［J］.J Leukocyte Biol,2011,89 (4):557-563.
［4］Wang L,Zhu MJ,Ren AM,et al.A ten-microRNA signature identified from a genome-wide microRNA expression profiling in human epithelial ovarian cancer［J］.PLoS One,2014,9(5):e96472.
［5］Wang YC,Li Y,Wang XY,et al.Circulating miR-130b mediates metabolic crosstalk between fat and muscle in overweight/obesity［J］.Diabetologia,2013,56(10):2275-2285.
［6］Gong X,Zhang K,Wang Y,et al.MicroRNA-130b targets Fmr1 and regulates embryonic neural progenitor cell proliferation and differentiation［J］.Biochem Biophys Res Commun,2013,439(4):493-500.
［7］Zhao G,Zhang JG,Shi Y,et al.MiR-130b is a prognostic marker and inhibits cell proliferation and invasion in pancreatic cancerthrough targeting STAT3［J］.PLoS One,2013,8(9):e73803.
［8］Colangelo T,Fucci A,Votino C,et al.MicroRNA-130b promotes tumor development and is associated with poor prognosis in colorectal cancer［J］.Neoplasia,2013,15(9):1086-1099.
［9］Li BL,Lu C,Lu W,et al.MiR-130b is an EMT-related microRNA that targets DICER1 for aggression in endometrial cancer［J］.Med Oncol,2013,30(1):484.
［10］Lai KW,Koh KX,Loh M,et al.MicroRNA-130b regulates the tumour suppressor RUNX3 in gastric cancer［J］.Eur J Cancer,.2010,46(8):1456-1463.
［11］Yeung ML,Yasunaga J,Bennasser Y,et al.Roles for microRNAs,miR-93 and miR-130b,and tumor protein 53-induced nuclear protein 1 tumor suppressor in cell growth dysregulation by human T-cell lymphotrophic virus 1［J］.Cancer Res,2008,68(21):8976-8985.
［12］Foey AD,S Crean.Macrophage subset sensitivity to endotoxin tolerisation by Porphyromonas gingivalis［J］.PLoS One,2013,8(7):e67955.
［13］Chanput W,Mes JJ,Savelkoul HF,et al.Characterization of polarized THP-1 macrophages and polarizing ability of LPS and food compounds［J］.Food Funct,2013,4(2):266-276.
［14］Moore CS,Rao VT,Durafourt BA,et al.miR-155 as a multiple sclerosis-relevant regulator of myeloid cell polarization［J］.Ann Neurol,2013,74(5):709-720.
［15］Banerjee S1,Xie N,Cui H,et al.MicroRNA let-7c regulates macrophage polarization［J］.J Immunol,2013,190(12):6542-6549.
［16］Yang J,Zhang Z,Chen C,et al.MicroRNA-19a-3p inhibits breast cancer progression and metastasis by inducing macrophage polarization through downregulated expression of Fra-1 proto-oncogene［J］.Oncogene,2014,33(23):3014-3023.

Memo

Memo:
辽宁省科技厅基金项目（编号：2013225021）
Last Update: 2015-02-02