|Table of Contents|

Folate deficiency inhibits colorectal cancer HCT-116 cell migration through the miR-5002-5p-Gadd45α axis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2025 04
Page:
548-556
Research Field:
Publishing date:

Info

Title:
Folate deficiency inhibits colorectal cancer HCT-116 cell migration through the miR-5002-5p-Gadd45α axis
Author(s):
CAO Yu1; 2; LU Lin3; ZHU Yuqian1; 4; GUO Xihan1; WANG Han1; WANG Xu1; 5; NI Juan1
1.College of Life Sciences,Yunnan Normal University,Yunnan Kunming 650500,China;2.Yunnan Cancer Institute,Yunnan Cancer Hospital,Yunnan Kunming 650118,China;3.Institute of Biomedical Engineering,Kunming Medical University,Yunnan Kunming 650500,China;4.Wuhan Kindstar Dlagnostlcs Co.,Ltd.,Hubei Wuhan 430075,China;5.Yeda Institute of Gene and Cell Therapy,Zhejiang Taizhou 318000,China.
Keywords:
folate deficiency; Gadd45α; miR-5002-5p; colorectal cancer; cell migration
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2025.04.002
Abstract:
Objective:To investigate whether FA deficiency alters the migration ability of colorectal cancer cells via the miR-5002-5p-Gadd45α axis.Methods:Dual luciferase reporter assay verified the targeting of miR-5002-5p and Gadd45α 3’ UTR.The expression of miR-5002-5p,Gadd45α and E-cadherin in colorectal cancer cell line (HCT-116) was detected by RT-qPCR and Western blot.Changes in cell migration were assessed using Transwell and scratch assays.Results:In HCT-116 cells,miR-5002-5p targeted and inhibited Gadd45α expression.Gadd45α expression was up-regulated by FA deficiency,and cell migration and miR-5002-5p expression were inhibited.Silencing Gadd45α or overexpressing miR-5002-5p inhibited the expression of Gadd45α and reversed the inhibitory effect of FA deficiency on cell migration.FA deficiency induced high expression of E-cadherin,and this effect was significantly inhibited after overexpression of miR-5002-5p.Conclusion:FA deficiency inhibits HCT-116 cell migration through the miR-5002-5p-Gadd45α axis,which is closely related to the change of epithelial mesenchymal status of the cells.Gadd45α is the molecular target in FA deficiency changing HCT-116 cell migration ability.

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Memo

Memo:
National Natural Science Foundation of China(No.31560307，31860301）；国家自然科学基金项目(编号：31560307，31860301)
Last Update: 1900-01-01