|Table of Contents|

PSMB9 regulates the PI3K/Akt signaling pathway to promote malignant biological behavior in esophageal squamous cell carcinoma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 24
Page:
4600-4607
Research Field:
Publishing date:

Info

Title:
PSMB9 regulates the PI3K/Akt signaling pathway to promote malignant biological behavior in esophageal squamous cell carcinoma
Author(s):
YAN Ke; ZHANG Zhouhua; LI Zhifang; WEI Xingfang; ZHANG Li
Department of Comprehensive Internal Medicine,the First Affiliated Hospital of Xinjiang Medical University,Xinjiang Urumqi 830000,China.
Keywords:
esophageal squamous cell carcinoma; PSMB9; PI3K/Akt signaling pathway
PACS:
R735.1
DOI:
10.3969/j.issn.1672-4992.2024.24.004
Abstract:
Objective:To explore the molecular function and possible molecular mechanisms of PSMB9 in the occurrence and development of esophageal squamous cell carcinoma.Methods:Collect the cancer and adjacent tissues of 54 patients with esophageal squamous cell carcinoma who underwent surgical treatment at the First Affiliated Hospital of Xinjiang Medical University from 2018 to 2022,and detect the protein expression level of PSMB9.Western blot was used to detect the protein expression level of PSMB9 in esophageal squamous cell carcinoma and normal esophageal epithelial cell lines.Transfection with lentivirus KY-SE150 and TE-13,RT-qPCR and Western blot were used to detect the mRNA and protein expression levels of PSMB9 in each group of cells after lentivirus transfection.Detect the proliferation,migration,invasion ability,and apoptosis rate of esophageal squamous cell carcinoma cells based on cell proliferation assay,cell scratch assay,Transwell assay,flow cytometry,and nude mouse subcutaneous tumorigenesis assay.Protein blot assay was used to detect the expression levels of key proteins in the PI3K/Akt signaling pathway.Results:Immunohistochemical results showed that the expression of PSMB9 was significantly increased in esophageal squamous cell carcinoma tissues compared to adjacent tissues (P＜0.05),and the high expression of PSMB9 was positively correlated with tumor size and lymph node metastasis.The cell experiment results showed that compared with the control group,knocking down PSMB9 significantly reduced the proliferation,migration,and invasion ability of esophageal squamous cell carcinoma cells,and significantly increased the apoptosis rate (P＜0.05).The in vivo experimental results showed that compared with the control group,the tumor volume and size of the knockdown group were significantly reduced,and the expression level of Ki-67 protein was significantly reduced (P＜0.05).Western blot analysis of key proteins in the PI3K/Akt signaling pathway revealed that the phosphorylation levels of p-PI3K and p-Akt proteins in the sh-PSMB9 group were significantly lower than those in the sh-NC group (P＜0.05).Conclusion:PSMB9,as a pro oncogene,affects the proliferation,migration,invasion ability,and apoptosis rate of esophageal squamous cell carcinoma cells,and its mechanism of action may be related to the PI3K/Akt signaling pathway.

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Memo

Memo:
新疆维吾尔自治区重点研发计划项目（编号：2020B03003-3，2020B03003）
Last Update: 1900-01-01