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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2024 24

Page:

4588-4599

Research Field:

Publishing date:

Info

Title:

Targeted degradation of p38¦Ã by hyperthermia inhibits proliferation of nasopharyngeal carcinoma cells

Author(s):

WU Jingjing¹; 2; LI Huizhen²; SHI Shuling²; LI Peihong¹; 2; YANG Yuchuan²; ZHA Boya²; HUANGFU Linkuan²; LIANG Tiansong²; ZHENG Yingjuan²; YANG Daoke²

1.School of Basic Medicine,Zhengzhou University,Henan Zhengzhou 450000£¬China;2.Radiotherapy Department£¬the First Affiliated Hospital of Zhengzhou University£¬Henan Zhengzhou 450000£¬China.

Keywords:

p38¦Ã; nasopharyngeal carcinoma; hyperthermia; proliferation

PACS:

R739.63

DOI:

10.3969/j.issn.1672-4992.2024.24.003

Abstract:

Objective:To investigate the effect of hyperthermia (HT) on nasopharyngeal carcinoma (NPC) cells proliferation and its mechanism.Methods:Water bath method was used to simulate HT.The proliferation of NPC cell line CNE-2 after 43 ¡æ HT£¬p38¦Ã gene knockout and overexpression were analyzed by CCK-8,EdU nuclear staining and clonal formation assay.Cell cycle parameters and apoptosis were detected by flow cytometry,p38¦Ã mRNA and protein expression levels were detected by qPCR and Western blot.Cell migration was detected by Transwell and scratch methods.Results:Similar to the downregulation of the p38¦Ã gene,high-temperature treated NPC cells showed growth inhibition associated with downregulation of p38¦Ã.And high temperatures were also found to block the cell cycle.However,the proliferation of cells with p38¦Ã overexpression was inhibited after HT treatment.Furthermore,high temperature increased the destruction of p38¦Ã-lysosome,leading to a decrease in the level of p38¦Ã protein in NPC.Bioinformatics analysis showed that down-regulation of p38¦Ã was the central node of the thermal response of NPC cells.Conclusion:High temperatures can easily disrupt the level of p38¦Ã NPC cells and inhibit tumor growth,effectively blocking the cycle.Therefore,hyperthermia is a new strategy to improve the therapeutic effectiveness of p38¦Ã-driven cancers.

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