|Table of Contents|

Lupalbigenin inhibits proliferation of NCI-H1975 cell in non-small cell lung cancer through the EGFR signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 09
Page:
1621-1626
Research Field:
Publishing date:

Info

Title:
Lupalbigenin inhibits proliferation of NCI-H1975 cell in non-small cell lung cancer through the EGFR signaling pathway
Author(s):
LI Siyu12HUANG Yanping1ZHU Weiwei12YANG Xingying12SHENG Jun1WANG Xuanjun1
1.Key Laboratory of Pu-er Tea Science and Technology,Yunnan Agricultural University,Yunnan Kunming 650201,China;2.College of Food Science and Techonology,Yunnan Agricultural University,Yunnan Kunming 650201,China.
Keywords:
LupalbigeninEGFRNCI-H1975 cellsproliferation inhibition
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2024.09.008
Abstract:
Objective:To investigate whether Lupalbigenin induces the proliferation of NCI-H1975 cells in non-small cell lung cancer(NSCLC) through epidermal growth factor receptor(EGFR) signaling pathway .Methods:MTT assay was used to detect cytotoxicity.Cell proliferation ability was observed and measured by cell morphology,cell migration and cell clonal formation.Western Blot was detected the effects Lupalbigenin of on EGFR and its downstream MAPK signaling pathway protein ERK and its phosphorylation levels,and the levels of apoptosis related proteins cleaved PARP,P21,CDK4,CDK6,CyclinD1,CyclinA2 and the levals of anti-apoptotic protein Bcl-2.Results:The IC50 value of Lupalbigenin in NCI-H1975 cell line was 3.246 μmol/L,and Lupalbigenin inhibited cell proliferation in a concentration-dependent way.The results of cell morphology showed that compared with the blank group,24 h treatment of 5 μmol/L Lupalbigenin experimental group resulted in a significant increase in nuclear shrinkage,cytoplasmic aggregationand cell apoptosis.Migration and colony formation experiments showed that 5 μmol/L LG could significantly inhibit cell migration and colony formation.Western Blot analysis showed that Lupalbigenin significantly inhibited the phosphorylation of EGFR/ERK protein in a short period of time.Meanwhile,after Lupalbigenin treatment,Cleaved PARP and P21 protein expression levels were significantly up-regulated,and CDK4,CDK6,CyclinD1,CyclinA2,and Bcl-2 protein expression levels were significantly decreased.Conclusion:In conclusion,Lupalbigenin can regulate the expression of EGFR and its downstream related proteins,thereby inhibiting the proliferation of NCI-H1975 cells.

References:

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Memo

Memo:
云南省基础研究专项青年项目(编号:202001AU070125);云南省农业联合专项面上项目(编号:202301BD070001-098)
Last Update: 2024-03-29