|Table of Contents|

Effect of fibroblast growth factor 19 stable knockdown on proliferation and migration of colorectal cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 09
Page:
1589-1594
Research Field:
Publishing date:

Info

Title:
Effect of fibroblast growth factor 19 stable knockdown on proliferation and migration of colorectal cancer cells
Author(s):
PENG Duo1; WANG Yan1; YANG Zisha2; HU Di2; HU Xinrong1; ZHAO Yi1
1.Basic Medical School,Guangdong Medical University,Guangdong Dongguan 523808,China;2.School of Medical Technology,Guangdong Medical University,Guangdong Dongguan 523808,China.
Keywords:
fibroblast growth factor 19; colorectal cancer; lentiviral vector; cell migration; cell proliferation
PACS:
R735.3+4
DOI:
10.3969/j.issn.1672-4992.2024.09.003
Abstract:
Objective:To investigate the effect of fibroblast growth factor 19(FGF19) knockdown on the proliferation and migration of colorectal cancer cells.Methods:The colorectal cancer cell lines COLO201 and HCT116 were transfected with lentivirus to achieve a stable knockdown of FGF19.The mRNA and protein expression of FGF19 were detected using PCR and Western blotting,respectively.Cell migration activity was assessed using the transwell assay,while cell proliferation was evaluated using the CCK-8 assay and colony formation assay.Results:Two colorectal cancer cell lines with stable knockdown of FGF19 were successfully established.The expression of FGF19 mRNA in the knockdown group was significantly lower than in the control group(P=0.001 1,P=0.000 1).Similarly,the expression of FGF19 protein was significantly decreased in the knockdown group compared to the control group(P=0.001,P=0.000 1).Moreover,the migration activity and proliferation ability of the knockdown group were significantly decreased compared to the control group(Transwell P=0.001 7/P=0.000 1,CCK-8 P=0.0425/P=0.033 4,Colon P=0.005 1/P=0.002 2).Conclusion:In conclusion,we successfully constructed a lentiviral vector for FGF19 knockdown and established colorectal cancer cell lines with stable knockdown of FGF19.Knockdown of FGF19 led to significantly reduced migration activity and proliferation of colorectal cancer cells.This study provides a basis for further investigation into the role of FGF19 in colorectal cancer.

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Memo

Memo:
National Natural Science Foundation of China（No.81572566）;国家自然科学基金资助项目(编号：81572566);广东省研究生教育创新计划项目（编号：2022XSLT047，2022SFKC063）
Last Update: 2024-03-29