|Table of Contents|

Regulation of LRG1 by TNF-ɑ promotes the proliferation,invasion and migration of breast cancer MCF-7 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 08
Page:
1424-1429
Research Field:
Publishing date:

Info

Title:
Regulation of LRG1 by TNF-ɑ promotes the proliferation,invasion and migration of breast cancer MCF-7 cells
Author(s):
GAO KexinLI WenyuanZHAO WeiZOU MingmingOU JinruiSUN Ping
Department of Anatomy,School of Basic Medicine,Mudanjiang Medical University,Heilongjiang Mudanjiang 157011,China.
Keywords:
TNF-αLRG1p38MAPKbreast cancer MCF-7 cells
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2024.08.009
Abstract:
Objective:To investigate the regulation of LRG1 protein expression by TNF-α in breast cancer MCF-7 cells and its effects on the proliferation,invasion and migration ability of MCF-7 cells.Methods:MTT assay was used to detect the viability of MCF-7 cells treated with different concentrations of TNF-α.Cell proliferation,invasion and migration capacity was assessed by EdU,Transwell and scratch experiments,respectively after inhibiting the expression of LRG1.Western blot was used to detect p-p38 protein expression in intracellular MAPK signaling pathway.Results:Breast cancer MCF-7 cells treated with low concentrations of TNF-α showed enhanced cell viability.Cell proliferation ability was decreased after inhibition of LRG1 expression.The number of invasive cells,migration rate and p-p38 protein expression were also decreased.Conclusion:TNF-ɑ promotes the proliferation,invasion,and migration of breast cancer MCF-7 cells by regulating LRG1 expression and this process may be achieved through the activation of the p38MAPK signaling pathway.

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黑龙江省基本科研业务费项目(编号:2022-KYYWF-0704);黑龙江省牡丹江市应用技术研究与开发计划(编号:HT2022JG133);牡丹江医学院导师科研专项计划(编号:YJSZX2022011)
Last Update: 1900-01-01