|Table of Contents|

Based on the differential expression of PKMYT1 in breast cancer,the influence of PKMYT1 on biological behavior of breast cancer was explored

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 07
Page:
1242-1249
Research Field:
Publishing date:

Info

Title:
Based on the differential expression of PKMYT1 in breast cancer,the influence of PKMYT1 on biological behavior of breast cancer was explored
Author(s):
AN Yanrong1; YUN Fen1; 2; SHI Lin1; 2; LIU Xia1; 2; JIA Yongfeng1; 2
1.Basic Medical College of Inner Mongolia Medical University,Inner Mongolia Hohhot 010030,China;2.Affiliated Hospital of Inner Mongolia Medical University,Inner Mongolia Hohhot 010050,China.
Keywords:
PKMYT1; breast cancer; bioinformatics; biological behavior
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2024.07.010
Abstract:
Objective:To clarify the effect of protein kinase membrane associated tyrosine/threonine 1(PKMYT1) on the biological behavior of breast cancer based on the differential expression of PKMYT1 in breast cancer,and to predict the effect of PKMYT1 on the prognosis of breast cancer by survival analysis.Methods:The expression of PKMYT1 in pancarcinoma and breast cancer was analyzed by bioinformatics to predict the effect of PKMYT1 on biological behavior of breast cancer.The Kaplan-Meier Plotter database was used to analyze the correlation between PKMYT1 and the prognosis of breast cancer.The effect of PKMYT1 on the biological behavior of breast cancer cell lines MCF-7 and MDA-MB-231 was verified by cell assay,and analyzed by CCK-8 and Transwell.The effects of PKMYT1 on the proliferation and invasion of MCF-7 and MDA-MB-231 cells were evaluated,and the effects of PKMYT1 on the cell cycle and apoptosis of breast cancer cells were detected by flow cytometry.Results:By bioinformatics analysis,PKMYT1 was highly expressed in a variety of tumor samples,including breast cancer,and the difference was statistically significant(P＜0.05).It was predicted that PKMYT1 expression was positively correlated with the cell cycle,DNA damage and repair,invasion,proliferation and apoptosis of breast cancer.Cell experiment results showed that PKMYT1 knockdown could inhibit the proliferation and invasion of MCF-7 and MDA-MB-231,and the increase of G0/G1 phase and decrease of S+G2/M phase in the detection of cell cycle indicated that the proliferation of breast cancer cells was inhibited and apoptosis was promoted.Survival analysis showed that PKMYT1's HR>1 in breast cancer was a high risk factor,and P＜0.05 indicated that PKMYT1 was closely associated with poor prognosis in breast cancer.Conclusion:PKMYT1 expression is up-regulated in breast cancer samples and is closely associated with poor prognosis.PKMYT1 may accelerate the malignant progression of breast cancer by regulating cell cycle.PKMYT1 is expected to become a potential therapeutic target for breast cancer.

References:

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Memo

Memo:
内蒙古自治区科技计划（编号：2019GG083）
Last Update: 2024-02-29