|Table of Contents|

Effects of SLC6A9 on the growth of colorectal cancer cells and the sensitivity to 5-FU

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 07
Page:
1236-1241
Research Field:
Publishing date:

Info

Title:
Effects of SLC6A9 on the growth of colorectal cancer cells and the sensitivity to 5-FU
Author(s):
ZHANG Yan; TIAN Suli; ZHOU Yongxu; LIU Chang
Department of General Surgery,the Fourth Affiliated Hospital of Harbin Medical University,Heilongjiang Harbin 150001,China.
Keywords:
colonic neoplasms; SLC6A9; cell migration and invasion analysis; EMT; 5-FU; xenotransplantation model experiment
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2024.07.009
Abstract:
Objective:To investigate the expression of member 9 of the solute carrier family 6(SLC6A9) on the proliferation,migration,and sensitivity of colon cancer cells to 5-fluorouracil(5-FU).Methods:TCGA database analysis,real-time fluorescence quantitative PCR,and Western blot analysis were used to detect the expression of SLC6A9 in colon cancer tissues,normal colon cell lines(NCM460),and colorectal cancer cell lines(SW620,HCT116,HT29,Lovo,and SW480).Transfect HT29 cells with SCL6A9 overexpression plasmids and negative controls(SLC6A9 OE,Vector),and transfect SW620 cells with SCL6A9 small interfering RNA and negative controls(SLC6A9 siRNA1#,siRNA2# and Scramble).The scratch healing experiment and transwell experiment were used to detect the migration and invasion ability of cells in each group.Western blot and cellular immunofluorescence were used to detect the expression levels of EMT related proteins E-cadherin and Vimentin.Using the CCK-8 method and constructing a nude mouse tumor model to detect the therapeutic effect of overexpression of SLC6A9 on 5-FU in colorectal cancer cells.Results:Compared with normal colon tissues and NCM460 cells,SLC6A9 was low expressed in colon cancer tissues and colorectal cancer cell lines(both P＜0.05).Overexpression of SLC6A9 leads to an increase in E-cadherin protein expression and a decrease in Vimentin protein levels,which inhibits the migration and invasion of colorectal cancer cells(P＜0.05).Low expression of SLC6A9 leads to a decrease in E-cadherin protein expression and an increase in Vimentin protein levels,promoting the migration and invasion ability of colorectal cancer cells(P＜0.05).Overexpression of SLC6A9 promoted the efficacy of 5-FU and resulted in slow tumor growth and reduced mass(P＜0.05).The low expression of SLC6A9 decreased the sensitivity of 5-FU(P＜0.05).Conclusion:Overexpression of SLC6A9 can inhibit the migration,invasion,and EMT progression of colorectal cancer cells,and enhance the sensitivity of 5-FU on colorectal cancer cells.

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Memo

Memo:
黑龙江省自然科学基金联合引导项目（编号：LH2019H079）
Last Update: 2024-02-29