|Table of Contents|

Lnc-PCIR promotes the proliferation and migration of laryngeal cancer cells by regulating the ubiquitination of PABPC4

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 07
Page:
1228-1235
Research Field:
Publishing date:

Info

Title:
Lnc-PCIR promotes the proliferation and migration of laryngeal cancer cells by regulating the ubiquitination of PABPC4
Author(s):
ZHANG Yang; DENG Xinxin; WANG Zhen; YANG Liping; ZHANG Yingying; LIANG Gengtian
Department of Otolaryngology,the Third Hospital of Wuhan,Tongren Hospital of Wuhan University,Hubei Wuhan 430060,China.
Keywords:
Lnc-PCIR; poly(A)-binding protein cytoplasmic 4; ubiquitination; laryngeal cancer
PACS:
R739.6
DOI:
10.3969/j.issn.1672-4992.2024.07.008
Abstract:
Objective:To explore the effect of LncRNA RP11-214F16.8(Lnc-PCIR) on the proliferation and migration of laryngeal cancer cells by regulating the ubiquitination of poly(A)-binding protein cytoplasmic 4(PABPC4).Methods:Use Human Protein Atlas and GEPIA databases to analyze Lnc-PCIR and PABPC4 levels in head and neck squamous cell carcinoma tissues and the overall survival of patients.Select laryngeal cancer and adjacent tissue samples from 130 laryngeal cancer patients in our hospital,RT-qPCR and Western blot were used to detect Lnc-PCIR,PABPC4 mRNA and protein levels,and the relationship between Lnc-PCIR level and clinical pathological parameters of patients was analyzed.HEP-2,TU212 cells were transfected differently,as si-Lnc-NC group,si-Lnc-PCIR group,Lnc-NC group,Lnc-PCIR group,si-Lnc-PCIR+NC group,si-Lnc-PCIR+PABPC4 group.CCK-8,EdU stainingand Transwell test were used to detect the proliferation and migration ability.RT-qPCR was used to detect the Lnc-PCIR,PABPC4 mRNA levels.HEP-2 and TU212 cells were treated with cycloheximide(CHX),MG132 and ML364,Western blot was used to detect PABPC4 protein level.20 nudes mice were subcutaneously injected with transfected HEP-2 cell suspension and recorded as si-Lnc-NC group and si-Lnc-PCIR group.After one month,the volume,mass,and Lnc-PCIR and PABPC4 protein levels of the transplanted tumor were measured.Results:Database showed that Lnc-PCIR and PABPC4 levels in head and neck squamous cell carcinoma tissue are higher than those in normal tissue,and Lnc-PCIR and PABPC4 levels were negatively correlated with overall survival(all P＜0.05).Compared with normal tissues,the mRNA and protein levels of Lnc-PCIR and PABPC4 were increased.Lnc-PCIR level was associated with gender,TNM stage,tumor differentiation,and lymph node metastasis(all P＜0.05).After knocking down Lnc-PCIR,Lnc-PCIR and PABPC4 protein levels in cells were decreased.The OD value of culturing 1 d,2 d and 3 d,EdU positive rate and the number of cell migration were decreased.Overexpression of PABPC4 could partially reverse the effects of knocking down Lnc-PCIR on cell proliferation and migration(all P＜0.05).Overexpression of Lnc-PCIR could reduce ubiquitination level of PABPC4(P＜0.05).Knocking down Lnc-PCIR could inhibit the growth of transplanted tumors in mice(P＜0.05).Conclusion:Lnc-PCIR promoted the proliferation and migration of laryngeal cancer cells by regulating the ubiquitination of PABPC4.

References:

［1］WANG Z,SUN AQ,YAN AH,et al.Circular RNA MTCL1 promotes advanced laryngeal squamous cell carcinoma progression by inhibiting C1QBP ubiquitin degradation and mediating beta-catenin activation［J］.Mol Cancer,2022,21(1):92.
［2］GAO K,ZHU YN,WANG H,et al.Network pharmacology reveals the potential mechanism of Baiying Qinghou decoction in treating laryngeal squamous cell carcinoma［J］.Aging(Albany NY),2021,13(24):26003-26021.
［3］吴玉湖,胡欣妍,杨宣叶,等.蛋白质泛素化修饰对流感病毒增殖和致病性的影响［J］.病毒学报,2023,39(4):1152-1160. WU YH,HU XY,YANG XY,et al.The effect of protein ubiquitination modification on the proliferation and pathogenicity of influenza viruses［J］.Chinese Journal of Virology,2023,39(4):1152-1160.
［4］HUANG S,ZHENG XY,ZHANG XY,et al.Exercise improves high-fat diet-induced metabolic disorder by promoting HDAC5 degradation through the ubiquitin-proteasome system in skeletal muscle［J］.Appl Physiol Nutr Metab,2022,47(11):1062-1074.
［5］MENG Y,SUN HY,LI YY,et al.Targeting ferroptosis by ubiquitin system enzymes:A potential therapeutic strategy in cancer［J］.Int J Biol Sci,2022,18(14):5475-5488.
［6］陈向宙,叶嘉慧,黎谢梦丹,等.长链非编码RNA RP11-214F16.8促进乳腺癌细胞增殖［J］.中国生物化学与分子生物学报,2018,34(8):900-904. CHEN XZ,YE JH,LI-XIE MD,et al.Long Non-coding RNA RP11-214F16.8 enhances proliferation of breast cancer cells［J］.Chinese Journal of Biochemistry and Molecular Biology,2018,34(8):900-904.
［7］LYU XX,ZHANG QY.LncRNA RP11-214F16.8 drives breast cancer tumorigenesis via a post-translational repression on NISCH expression［J］.Cell Signal,2022,92:110271.
［8］KOSTIANETS O,ANTONIUK S,FILONENKO V,et al.Immunohistochemical analysis of medullary breast carcinoma autoantigens in different histological types of breast carcinomas［J］.Diagn Pathol,2012,7:161.
［9］LIU D,YIN B,WANG Q,et al.Cytoplasmic poly(A)binding protein 4 is highly expressed in human colorectal cancer and correlates with better prognosis［J］.J Genet Genomics,2012,39:369-374.
［10］HSU CH,HSU CW,HSUEH C,et al.Identification and characterization of potential biomarkers by quantitative tissue proteomics of primary lung adenocarcinoma［J］.Mol Cell Proteomics,2016,15:2396-2410.
［11］JIANG X,WANG GG,LIU YY,et al.A novel long non-coding RNA RP11-286H15.1 represses hepatocellular carcinoma progression by promoting ubiquitination of PABPC4［J］.Cancer Lett,2021,499:109-121.
［12］GUO WH,LI JY,HUANG HB,et al.LncRNA PCIR is an oncogenic driver via strengthen the binding of TAB3 and PABPC4 in triple negative breast cancer［J］.Front Oncol,2021,11:630300.
［13］MENG K,LU SH,LI YY,et al.LINC00493-encoded microprotein SMIM26 exerts anti-metastatic activity in renal cell carcinoma［J］.EMBO Rep,2023,24(6):e56282.
［14］WANG QD,LIU LL,LI D,et al.Salt-like transcription factor 4 promotes laryngeal cancer progression through transcriptional activation of ubiquitin-specific protease 21 to stabilize Yin Yang 1［J］.Pathol Int,2023,73(3):109-119.
［15］LIU XY,KUMAR M,YANG LP,et al.BAP1 is a novel target in HPV-negative head and neck cancer［J］.Clin Cancer Res,2018,24(3):600-607.

Memo

Memo:
湖北省武汉市卫生和计划生育委员会科研项目（编号：WZ16Z13）
Last Update: 2024-02-29