|Table of Contents|

Ultrasound microbubble mediated miR-545-3p inhibits the malignant progression of breast cancer by regulating SEPT2

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 07
Page:
1222-1228
Research Field:
Publishing date:

Info

Title:
Ultrasound microbubble mediated miR-545-3p inhibits the malignant progression of breast cancer by regulating SEPT2
Author(s):
LI Wenjing; ZHANG Qiang
Department of Ultrasound,Jingzhou Hospital Affiliated to Yangtze University,Hubei Jingzhou 434020,China.
Keywords:
ultrasound microbubbles; miR-545-3p; SEPT2; breast cancer; malignant progression
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2024.07.007
Abstract:
Objective:To study the mechanism of miR-545-3p mediated by ultrasound microbubbles in inhibiting the malignant progression of breast cancer by regulating septin 2(SEPT2).Methods:The expression of miR-545-3p and SEPT2 in human normal breast epithelial cells MCF-10A,human breast cancer cells MCF-7,HS-578T and HCC-1937 cells were detected by real-time fluorescent quantitative PCR and Western blot.MCF-7 cells were cultured in vitro and randomly grouped into control group,empty microbubble group,miR-545-3p microbubble group,and miR-545-3p+SEPT2 overexpression microbubble group,after grouping and processing,real-time fluorescence quantitative PCR and immunoblotting experiments were applied to detect the expression of miR-545-3p and SEPT2 of cells in each group.CCK-8,colony formation assay,and flow cytometry were applied to detect cell proliferation and apoptosis in each group.Cell scratch and Transwell invasion tests were applied to detect cell migration and invasion in each group.Nude mice were subcutaneously inoculated with MCF-7 cells on the abdominal wall to construct their transplanted tumor model and grouped using the same method.After grouping,the tumor volume and weight of each group of nude mice were measured.Results:Compared with MCF-10A cells,the expression of miR-545-3p in MCF-7,HS-578T,and HCC-1937 cells decreased(P＜0.05),the expression of SEPT2 mRNA and protein increased(P＜0.05).Compared with the control group,the miR-545-3p expression and apoptosis rate in the miR-545-3p microbubble group increased(P＜0.05),the cell SEPT2 mRNA and protein expression,cell viability,colony formation rate,migration rate,invasion number,and tumor volume and weight in nude mice reduced(P＜0.05).Compared with the miR-545-3p microbubble group,the cell apoptosis rate in the miR-545-3p+SEPT2 overexpression microbubble group decreased(P＜0.05),the cell SEPT2 mRNA and protein expression,cell viability,colony formation rate,migration rate,invasion number,and tumor volume and weight in nude mice increased(P＜0.05).Conclusion:Ultrasound microbubble-mediated miR-545-3p can reduce the proliferation,migration and invasion of breast cancer cells,promote their apoptosis,inhibit the growth of transplanted tumor in nude mice,and finally inhibit the malignant progression of breast cancer cells by reducing the expression of SEPT2.

References:

［1］SARHANGI N,HAJJARI S,HEYDARI SF,et al.Breast cancer in the era of precision medicine［J］.Mol Biol Rep,2022,49(10):10023-10037.
［2］吴国泰,马倩倩,陈艳娥,等.乳腺癌血行肺转移模型家兔血小板与凝血功能变化研究［J］.中国实验动物学报,2022,30(6):784-791. WU GT,MA QQ,CHEN YE,et al.Changes of platelet and coagulation function in rabbits with hematogenous lung metastasis of breast cancer［J］.Chinese Journal of Experimental Animal,2022,30(6):784-791.
［3］GAO Y,LIU C,XU X,et al.Circular RNA sterile alpha motif domain containing 4A contributes to cell 5-fluorouracil resistance in colorectal cancer by regulating the miR-545-3p/6-phosphofructo-2-kinase/fructose-2,6-bisphosphataseisotype 3 axis［J］.Anticancer Drugs,2022,33(6):553-563.
［4］ZHANG Y,YU Y,CAO X,et al.Role of lncRNA FAM83H antisense RNA1(FAM83H-AS1) in the progression of non-small cell lung cancer by regulating the miR-545-3p/heparan sulfate 6-O-sulfotransferase(HS6ST2) axis［J］.Bioengineered,2022,13(3):6476-6489.
［5］WANG X,SONG H,FANG L,et al.EIF4A3-mediated circPRKCI expression promotes triple-negative breast cancer progression by regulating WBP2 and PI3K/AKT signaling pathway［J］.Cell Death Discov,2022,8(1):92-105.
［6］THOUDAM T,CHANDA D,SINAM IS,et al.Noncanonical PDK4 action alters mitochondrial dynamics to affect the cellular respiratory status［J］.Proc Natl Acad Sci USA,2022,119(34):e2120157119-e2120157129.
［7］YANG Z,ZHOU L,SI T,et al.Lysyl hydroxylase LH1 promotes confined migration and metastasis of cancer cells by stabilizing Septin2 to enhance actin network［J］.Mol Cancer,2023,22(1):21-38.
［8］CAI B,WANG X,BU Q,et al.LncRNA AFAP1-AS1 knockdown represses cell proliferation,migration,and induced apoptosis in breast cancer by downregulating SEPT2 via sponging miR-497-5p［J］.Cancer Biother Radiopharm,2022,37(8):662-672.
［9］FANG F,XU W,ZHANG J,et al.Ultrasound microbubble-mediated RNA interference targeting WNT1 inducible signaling pathway protein 1(WISP1) suppresses the proliferation and metastasis of breast cancer cells［J］.Bioengineered,2022,13(4):11050-11060.
［10］CHEN JL,LIN CY,LAI MY,et al.Effect of Caprin-1-KO transfection mediated by ultrasonic microvesicle on proliferation,cycle and expression of cyclinD1 and cyclinD2 in human hepatocellular carcinoma HepG2 cells［J］.Journal of Practical Medicine,2021,37(10):1250-1256.
［11］李延辉,方茅,谢敏如,等.慢病毒介导eIF3b-shRNA对裸鼠MCF-7乳腺癌移植瘤的抑制作用［J］.现代肿瘤医学,2022,30(15):2722-2726. LI YH,FANG M,XIE MR,et al.Lentivirus mediated eIF3b shRNA inhibits the transplanted tumor of MCF-7 breast cancer in nude mice［J］.Modern Oncology,2022,30(15):2722-2726.
［12］罗小琴,刘朝奇,邹云雷,等.超声介导载阿霉素微泡对小鼠H22肝癌皮下移植瘤抑制作用的实验研究［J］.中国超声医学杂志,2022,38(1):111-115. LUO XQ,LIU CQ,ZOU YL,et al.Experimental study on the inhibitory effect of ultrasound mediated doxorubicin loaded microbubbles on subcutaneous transplanted H22 liver cancer in mice［J］.Chinese Journal of Ultrasound Medicine,2022,38(1):111-115.
［13］KAWIAK A.Molecular research and treatment of breast cancer［J］.Int J Mol Sci,2022,23(17):9617-9619.
［14］KUNKLER IH,WILLIAMS LJ,JACK WJL,et al.Breast-conserving surgery with or without irradiation in early breast cancer［J］.N Engl J Med,2023,388(7):585-594.
［15］JIANG LP,WANG X,PENG XH.Effects of miR-34a-5p on biological behavior of breast cancer cells by targeting IMP3 and PD-L1［J］.Chinese Journal of Comparative Medicine,2023,33(7):67-77.
［16］王贝，刘锋.乳腺癌新辅助内分泌治疗与靶向治疗联合应用研究进展［J］.现代肿瘤医学，2022，30（21）：3996-4000. WANG B,LIU F.Research progress of neoadjuvant endocrine therapy combined with targeted therapy for breast cancer［J］.Modern Oncology，2022，30（21）：3996-4000.
［17］田甜，李尚子砚，庞慧.乳腺癌内分泌耐药机制及治疗对策的研究进展［J］.现代肿瘤医学，2022，30（18）：3436-3440. TIAN T,LI SZY,PANG H.Research progress in endocrine resistance mechanism and treatment of breast cancer［J］.Modern Oncology，2022，30（18）：3436-3440.
［18］SUN C,GUAN H,LI J,et al.circ_0000376 knockdown suppresses non-small cell lung cancer cell tumor properties by the miR-545-3p/PDPK1 pathway［J］.Open Med(Wars),2023,18(1):20230641-20230652.
［19］SUN H,LIU F,ZHANG H.Circ_0072008,an oncogene in pancreatic ductal adenocarcinoma,contributes to tumour cell malignant progression and glycolysis by regulating miR-545-3p/SLC7A11 axis［J］.Autoimmunity,2022,55(3):203-213.
［20］于姣姣，査莉，朱艳艳.超声微泡介导miRNAs在肿瘤治疗中的应用进展［J］.现代肿瘤医学，2022，30（18）：3415-3418. YU JJ,ZHA L,ZHU YY.Application of ultrasound microbubble mediated miRNAs in tumor therapy［J］.Modern Oncology，2022，30（18）：3415-3418.
［21］ZHANG XY,LIU ZX,ZHANG YF,et al.SEPT2 crotonylation promotes metastasis and recurrence in hepatocellular carcinoma and is associated with poor survival［J］.Cell Biosci,2023,13(1):63-78.
［22］ZHANG J,WANG M,WANG J,et al.JMJD2C-mediated long non-coding RNA MALAT1/microRNA-503-5p/SEPT2 axis worsens non-small cell lung cancer［J］.Cell Death Dis,2022,13(1):65-73.
［23］DEVLIN L,OKLETEY J,PERKINS G,et al.Proteomic profiling of the oncogenic septin 9 reveals isoform-specific interactions in breast cancer cells［J］.Proteomics,2021,21(19):e2100155-e2100188.

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Last Update: 2024-02-29