|Table of Contents|

M2 tumor-associated macrophages promote the proliferation,invasion and migration of lung cancer cells by regulating energy metabolism reprogramming

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 07
Page:
1215-1221
Research Field:
Publishing date:

Info

Title:
M2 tumor-associated macrophages promote the proliferation,invasion and migration of lung cancer cells by regulating energy metabolism reprogramming
Author(s):
QIU Youzheng1; SONG Zheng2; YANG Lijie2; LI Jun1; WANG Jing1
1.Department of Clinical Medicine,Dali University,Yunnan Dali 671000,China;2.Cardio-Thoracic Surgery,the First Affiliated Hospital of Dali University,Yunnan Dali 671000,China.
Keywords:
lung cancer; cell proliferation; tumor-associated macrophages; tumor immune microenvironment; metabolic reprogramming
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2024.07.006
Abstract:
Objective:To investigate the effects of M2 tumor-associated macrophages(TAM) on proliferation,invasion,migration and energy metabolic reprogramming of lung cancer cells.Methods:M2-type macrophages were induced by stimulation of 100 ng/mL phorbol 12-myristate 13-acetate(PMA)+IL-4 (20 ng/mL)+IL-13(20 ng/mL) for 48 h with final concentration of 320 ng/mL PMA.The expressions of iNOS,IL-1β,TNF-α,Arg-1,CCL-18 and IL-10 were detected by qRT-PCR,and the expressions of CD206 and CD86 were detected by immunofluorescence.THP-1 macrophage conditioned medium(M0-CM) and M2-type macrophage conditioned medium(M2-CM) derived from THP-1 polarization were collected,and lung cancer cells(A549 and H1299) were treated with M0-CM and M2-CM for 48 h,and the changes in cell proliferation capacity were detected by EdU.Annexin V-FITC/PI double staining was used to detect apoptosis of lung cancer cells.Transwell was used to detect cell invasion and migration.Energy metabolism of lung cancer cells was detected by LC-MS/MS.Results:Compared with M0,the expressions of iNOS(P＜0.01),IL-1β(P＜0.01) and TNF-α(P＜0.01) in M2-type macrophages were significantly decreased,while the expressions of Arg-1(P＜0.01),CCL-18(P＜0.01) and IL-10(P＜0.01) were significantly increased.The expression of surface antigen CD206 was significantly increased,while the expression of CD86 was significantly decreased.Compared with M0-CM,M2-CM could significantly promote the proliferation of lung cancer cells(A549,H1299)(P＜0.01),inhibit the apoptosis of lung cancer cells(A549,H1299)(P＜0.01),and promote the invasion and migration of lung cancer cells(A549,H1299)(P＜0.01).Compared with M0-CM,M2-CM can significantly promote the contents of D-xylose 5-phosphate,phosphoethanolamine,uridine 5-monophosphate,inosine-1-phosphate,adenosine acid,glucose,D-ribulose 5-phosphate,6-phosphogluconic acid,fructose 1,6-diphosphate,2-phospho-D-glyceric acid,etc.The contents of glutamine,tyrosine,leucine,lysine and threonine were reduced.KEGG enrichment analysis showed that the differential metabolites were mainly concentrated in amino acid biosynthesis,amino acid tRNA biosynthesis,mineral absorption,protein degradation and absorption.Conclusion:M2 tumor-associated macrophages can promote the proliferation,invasion and migration of lung cancer cells and inhibit the apoptosis of lung cancer cells.The mechanism may be related to the regulation of energy metabolic reprogramming of lung cancer cells.

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Memo

Memo:
大理大学第一附属医院临床医学学科建设重点项目（编号：DFYZD2022-07）;大理大学第一附属医院杰出中青年人才项目（编号：DFYJC-202107）
Last Update: 2024-02-29