|Table of Contents|

HOTTIP regulates the expression of Vimentin gene through competitively binding miR-506 to regulate the migration of clear cell renal cell carcinoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 07
Page:
1200-1207
Research Field:
Publishing date:

Info

Title:
HOTTIP regulates the expression of Vimentin gene through competitively binding miR-506 to regulate the migration of clear cell renal cell carcinoma cells
Author(s):
WANG Xiaoling; MENG Lidan; WANG Xuemin; ZHANG Yingxuan; ZHANG Chengguo
Department of Nephrology,People's Hospital of Hengshui,Hebei Hengshui 053000,China.
Keywords:
HOTTIP; miR-506; Vimentin; ccRCC; migration
PACS:
R737.11
DOI:
10.3969/j.issn.1672-4992.2024.07.004
Abstract:
Objective:To explore the mechanism of HOTTIP on regulating the expression of Vimentin gene to regulate the migration of clear cell renal cell carcinoma(ccRCC) cells through competitively binding miR-506.Methods:Bioinformatics analysis was performed on differentially expressed miRNA,mRNA and lncRNA of ccRCC in The Cancer Genome Atlas database.miRcode database was used to predict the binding of lncRNA-miRNA.The target gene regulated by miRNA was also predicted.The expression of HOTTIP,miR-506 and Vimentin mRNA in ccRCC cell lines were detected by qRT-PCR.The binding of miR-506 and HOTTIP was detected by RNA pull-down assay.The binding of miR-506 and HOTTIP or Vimentin was detected by RNA binding protein immunoprecipitation assay.The migratory ability of ccRCC 786-O cells was detected by Transwell and wound healing assays.Results:HOTTIP was significantly upregulated in ccRCC and significantly related to the prognosis of patients.The target miRNA of HOTTIP(miR-506) was markedly downregulated in ccRCC.Downstream regulatory gene Vimentin of miR-506 was significantly upregulated in ccRCC,and significantly related to the prognosis of patients.HOTTIP regulated the expression of Vimentin gene through competitively binding miR-506.Cell migratory ability was significantly decreased after interfering with HOTTIP.However,the inhibitory effect of cell migratory ability by interfering with HOTTIP could be reversed by silencing miR-506 or overexpressing Vimentin simultaneously.Conclusion:HOTTIP could regulate the expression of Vimentin gene to regulate the migration of ccRCC through competitively binding miR-506.

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Last Update: 2024-02-29