|Table of Contents|

Mechanism of dasatinib regulating biological behaviors of breast cancer cells based on PI3K/AKT signaling pathways

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 07
Page:
1194-1199
Research Field:
Publishing date:

Info

Title:
Mechanism of dasatinib regulating biological behaviors of breast cancer cells based on PI3K/AKT signaling pathways
Author(s):
SHEN Yunyan; QIU Qi
Outpatient Office,the Second Affiliated Hospital of Soochow University,Jiangsu Suzhou 215004,China.
Keywords:
dasatinib; breast cancer; PI3K/AKT signaling pathway; cell proliferation; apoptosis; migration; invasion
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2024.07.003
Abstract:
Objective:To explore the effects of dasatinib(DAS) on biological behaviors of breast cancer MCF-7 cells based on PI3K/AKT signaling pathways.Methods:The proliferation,invasion,migration and apoptosis of MCF-7 cells,and expressions of PI3K/AKT signaling pathway related proteins after treatment with different concentrations of DAS(0,2,6,10 μmol/L) were detected by methyl thiazolyl tetrazolium(MTT) assay,Transwell assay,flow cytometry and Western blotting,respectively.The control group(solvent control),DAS group(DAS 10 μmol/L),PI3K inhibitor group(LY294002 20 μmol/L) and combination group(DAS 10 μmol/L+LY294002 20 μmol/L) were set up.The cells proliferation,invasion,migration,apoptosis and expressions of PI3K/AKT signaling pathway related proteins were compared among different groups.Results:With the increase of DAS concentration,proliferation inhibition rate and apoptosis rate of MCF-7 cells were increased(P＜0.05),while number of invasion and migration cells,and expressions of PI3K,p-PI3K and p-AKT were decreased(P＜0.05).Compared with control group,proliferation inhibition rate and apoptosis rate of MCF-7 cells were increased(P＜0.05),while expressions of PI3K,p-PI3K and p-AKT were decreased in the other three groups.Compared with PI3K inhibitor group and DAS group,proliferation inhibition rate and apoptosis rate of MCF-7 cells were increased,while expressions of PI3K,p-PI3K and p-AKT were decreased in combination group(P＜0.05).Conclusion:DAS can inhibit proliferation,invasion and migration of breast cancer MCF-7 cells,and induce apoptosis.The mechanism may be related to the regulation of PI3K/AKT signaling pathways.

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Memo

Memo:
江苏省卫生健康委员会科研项目（编号：JJ2019M066）；江苏省临床医学科技专项（编号：BL2017044）
Last Update: 2024-02-29