|Table of Contents|

Mechanism of rhizoma curcumae-rhizoma sparganii against hepatocellular carcinoma based on PD-1 mediated NF-κB pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 07
Page:
1179-1185
Research Field:
Publishing date:

Info

Title:
Mechanism of rhizoma curcumae-rhizoma sparganii against hepatocellular carcinoma based on PD-1 mediated NF-κB pathway
Author(s):
GUO Honglin1; 2; 3; LI Jing4; ZHANG Qi1; CUI Wenyu1; JIANG Xicheng5; LI Wenlan1; 2; 3; ZHANG Qiao1; 2; 3
1.School of Pharmacy,Harbin University of Commerce,Heilongjiang Harbin 150076,China;2.National Ministry of Education Antitumor Natural Drug Engineering Research Center，Heilongjiang Harbin 150076,China;3.Heilongjiang Province Primary Laboratory of Drug Research for Prevention and Treatment of Senile Diseases,Heilongjiang Harbin 150076,China;4.Harbin Nebula Medical Laboratory,Heilongjiang Harbin 150028,China；5.Heilongjiang University of Chinese Medicine，Heilongjiang Harbin 150040，China.
Keywords:
rhizoma curcumae-rhizoma sparganii; liver cancer; NF-κB; PD-1
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2024.07.001
Abstract:
Objective:Based on programmed death-1(PD-1)-mediated NF-κB pathway,the effect of rhizoma curcumae-rhizoma sparganii on hepatocellular carcinoma cells and its mechanism were explored.Methods:In vivo,the effects of each group of drugs on tumor inhibition rate and organ index of H22 liver cancer-bearing mice were detected.The effects of drugs in each group on the levels of interleukin-1(IL-1), interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) were detected.Immunohistochemistry was used to observe the positive expression of NF-κBp65,Bcl-2 and Bax protein in tumor tissues.MTT assay was used to detect the inhibitory rate of curcumol and trigonolactone B alone and in combination on HepG2 liver cancer cells.Western blot was used to detect the effect of combination of drugs on PD-1,NF-κB,Bcl-2, κB kinase α/β(IKK-α/β) and nuclear factor κB inhibitor α(IκB-α).Results:In vivo experiments showed that compared with the model group,the combination of drugs could significantly reduce the tumour weight,could inhibit the expression of TNF-α,IL-1 and IL-6,of which TNF-α,IL-1 and IL-6 decreased significantly in the high-dose group of the combination of drugs,and could inhibit the positive cellular expression rate of NF-κBp65,Bcl-2 protein,and high expression of Bax protein in the tumour tissue.In vitro experiments showed that compared with the model group,the co-administration group could significantly inhibit the expression of PD-1,Bcl-2 and IKK-α/β proteins and promote the expression of NF-κB and IκB-α proteins.Conclusion:The combination of drugs can significantly inhibit the growth of tumours,inhibit the secretion of inflammatory factors,reduce or elevate the expression of NF-κB pathway-related proteins,and antagonize the proliferation of hepatocellular carcinoma cells in loaded mice.The combined application of curcumol and sparganium lactone B can improve the proliferation inhibition rate of HepG2 cells,which may be related to the regulation of NF-κB pathway mediated by PD-1.

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Memo

Memo:
National Natural Science Foundation of China（No.82174261）;国家自然科学基金（编号：82174261）；黑龙江省中医药管理局中医药项目（编号：ZHY2022-046）
Last Update: 2024-02-29