|Table of Contents|

PTPRJ promotes the adhesion,migration and invasion of prostate cancer cells through Src/PI3K/Akt signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

2023 13
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PTPRJ promotes the adhesion,migration and invasion of prostate cancer cells through Src/PI3K/Akt signaling pathway
ZHU Baoan1ZHENG Jianxin2ZHANG Yinpo3
1.Department of Biochemistry,Luohe Medical College,Henan Luohe 462002,China;2.The Second Hospital to Luohe Medical College,Henan Luohe 462002,China;3.Pathological Examination Center,Luohe Medical College,Henan Luohe 462002,China.
protein tyrosine phosphatase receptor type Jprostate cancer cell DU145Src/PI3K/Akt signaling pathway
Objective:To investigate the effects of PTPRJ gene expression on the adhesion,migration and invasion of DU145 cells of prostate cancer and the possible regulatory mechanism.Methods:Real-time quantitative PCR and Western blot were used to detect the expression of PTPRJ in prostate tumor tissues and cell lines.PTPRJ expression was silenced by infecting cells with recombinant lentivirus(LV-shPTPRJ) carrying PTPRJ-specific shRNA.Cell adhesion was detected by MTT,cell migration and invasion were detected by Transwell.Real-time quantitative PCR and Western blot were used to detect the mRNA and protein expression of signaling pathway molecules.Results:Compared with normal prostate tissues and cells,PTPRJ expression was increased in prostate tumor tissues and PC-3 and DU145 cell lines(P<0.05).Compared with the control group,the adhesion,migration and invasion ability of DU145 cells were significantly decreased after PTPRJ silencing(P<0.01),and the expression levels of signaling pathway proteins pY418Src,p-PI3K and p-Akt were significantly decreased(P<0.05).Activation of PI3K/Akt by SC79 reversed the effect of PTPRJ downregulation on DU145 cell adhesion and invasion.Silencing PTPRJ down-regulated the expression of pY418Src,p-PI3K and p-Akt in the tumor tissue of nude mice(P<0.05).Conclusion:PTPRJ may promote the adhesion,migration and invasion of DU145 cells by activating the Src/PI3K/Akt signaling pathway,indicating that PTPRJ may be a potential target for prostate cancer treatment.


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Last Update: 2023-05-31