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Influences of LncRNA FENDRR on the proliferation,epithelial-mesenchymal transition and invasion of esophageal cancer cells by regulating the expression of miR-942-5p

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

2023 13
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Influences of LncRNA FENDRR on the proliferation,epithelial-mesenchymal transition and invasion of esophageal cancer cells by regulating the expression of miR-942-5p
YANG Hailong1WANG Dingding1CHEN Yutong1CHEN Xiaowei1LI Bing1WANG Xinli2
1.Department of Thoracic Surgery;2.Department of Neurology,Harrison International Peace Hospital,Hebei Hengshui 053000,China.
long non-coding RNA FENDRRmicroRNA-199a-5pesophageal cancerproliferationepithelial-mesenchymal transitioninvasion
Objective:To investigate the effect of lncRNA FENDRR on EC cell proliferation,epithelial-mesenchymal transition(EMT) and invasion via miR-942-5p.Methods:EC and esophageal normal tissues were collected,and EC-113 cells were separated into BC group(without transfection),overexpression(OE)-FENDRR-NC group(transfected with pcDNA empty plasmid),OE-FENDRR group(transfected with pcDNA-LncRNA FENDRR plasmid),OE-FENDRR+miR-942-5p mimics-NC group(co-transfected with pcDNA-LncRNA FENDRR+miR-942-5p mimics-NC) and OE-FENDRR+miR-942-5p mimics group(co-transfection of pcDNA-LncRNA FENDRR+miR-942-5p mimics).Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR) was performed to analyze LncRNA FENDRR and miR-942-5p expression in tissues or cells.Cell counting kit-8 (CCK-8) method,nude mouse tumorigenic experiment and Transwell method were performed to analyze the proliferation and invasion activities of EC-113 cells in vitro and in vivo.Dual-luciferase reporter assay and RNA pull down assay were performed to analyze the targeting relationship between LncRNA FENDRR and miR-942-5p.Western blot was performed to analyze EMT-related protein expression.Results:Transfection of pcDNA-LncRNA FENDRR plasmid was able to increase the expression of LncRNA FENDRR in EC-113 cells,decrease the expression of miR-942-5p,and decrease the proliferation activity and invasion number in vitro and in vivo,levels of N-cadherin(N-CAD),vimentin(VIM),snial and matrix metalloproteinase 9(MMP9) proteins,and increase the E-cadherin(E-CAD) protein level(P<0.05),while miR-942-5p mimics reversed the above effects.LncRNA FENDRR was able to target and downregulate miR-942-5p expression.Conclusion:LncRNA FENDRR is a miR-942-5p molecular sponge,which can down-regulate the expression of miR-942-5p,thereby inhibiting proliferation,EMT and invasion behavior of EC cells.


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Last Update: 2023-05-31