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Effect of overexpression of lnc-TNFAIP3 gene on biological behavior of pancreatic cancer PANC-1 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

2023 13
Research Field:
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Effect of overexpression of lnc-TNFAIP3 gene on biological behavior of pancreatic cancer PANC-1 cells
HU Xianhua1ZHANG Rendan2YAO Jiaxin1ZHAO Chunyan3MU Bo4
1.Department of Laboratory Medicine;2.Department of Stomatology;3.Key Laboratory of Medical Imaging;4.School of Basic Medicine and Forensic Medicine,North Sichuan Medical College,Sichuan Nanchong 637000,China.
lentivirustransfectionlnc-TNFAIP3PANC-1pancreatic cancer
Objective:To construct pancreatic cancer cell lines with lnc-TNFAIP3 gene overexpression by lentivirus transfection and observe the changes of cell behavior.Methods:The overexpressed plasmid and lentivirus were prepared and transfected into human pancreatic cancer cell line PANC-1.The cell proliferation was detected by CCK-8 and cloning assay,the migration and invasion were detected by scratch and Transwell,the protein expression was detected by Western blot,and the cell cycle and apoptosis were detected by flow cytometry.Results:PANC-1 were successfully transfected with lentivirus overexpressing lnc-TNFAIP3 gene,and the proliferation,migration and invasion of PANC-1 were decreased.There was no significant difference in cell cycle and apoptosis,and no significant difference in the expression of related proteins.Conclusion:Human pancreatic cancer cell line PANC-1 constructed by lentivirus transfection with lnc-TNFAIP3 gene overexpression has changed in cell behavior,the present results suggest that lnc-TNFAIP3 may be a promising therapeutic target for pancreatic cancer,which provide a theoretical and experimental data for the study of lnc-TNFAIP3 gene in pancreatic cancer.


[1]MOORE A,DONAHUE T.Pancreatic cancer[J].JAMA,2019,322(14):1426.
[2]ALISON PK.Pancreatic cancer epidemiology:understanding the role of lifestyle and inherited risk factors[J].Nat Rev Gastroenterol Hepatol,2021,18(7):493-502.
[3]GUO W,DONG ZM,SHI YB,et al.Aberrant methylation-mediated downreguLation of long noncoding RNA LOC100130476 correlates with malignant progression of esophageal squamous cell carcinoma[J].Dig Liver Dis,2016,48(8):961-969.
[4]GUO W,DONG ZM,SHI YB,et al.Methylation-mediated downregulation of long noncoding RNA LOC100130476 in gastric cardia adenocarcinoma[J].Clin Exp Metastasis,2016,33(5):497-508.
[5]HERTER EK,LI D,TOMA MA,et al.WAKMAR2,a long noncoding rnadownregulated in human chronic wounds,modulates keratinocyte motility and production of inflammatory chemokines[J].J Invest Dermatol,2019,139(6):1373-1384.
[6]WANG LB,LIU JK,TAI JT,et al.A prospective study revealing the role of an immune-related eRNA,WAKMAR2,in breast cancer[J].Sci Rep,2021,11(1):15328-15330.
[7]ZHOU XP,XIE DL,HUANG J,et al.Therapeutic effects of (5R)-5-hydroxytriptolide on fibroblast-like synoviocytes in rheumatoid arthritis via lncRNA WAKMAR2/miR-4478/E2F1/p53 axis[J].Front Immunol,2021,12:605616.
[8]EGUCHI H,KOBAYASHI S,GOTOH K,et al.Characteristics of early-onset pancreatic cancer and its association with familial pancreatic cancer and hereditary pancreatic cancer syndromes[J].Ann Gastroenterol Surg,2020,4(3):229-233.
[9]CAI J,CHEN HD,LU M,et al.Advances in the epidemiology of pancreatic cancer:Trends,risk factors,screening,and prognosis[J].Cancer Lett,2021,520:1-11.
[10]XIE WK,CHU M,SONG GD,et al.Emerging roles of long noncoding RNAs in chemoresistance of pancreatic cancer[J].Semin Cancer Biol,2022,83:303-318.
[11]QIAO YN,JIN T,GUAN SD,et al.Long non-coding RNA Lnc-408 promotes invasion and metastasis of breast cancer cell by regulating LIMK1[J].Oncogene,2021,40(24):4198-4213.
[12]KEISUKE K,BONGYONG L,HARITHA K,et al.The long noncoding RNA lnc-HLX-2-7 is oncogenic in group 3 medulloblastomas[J].Neuro Oncol,2021,23(4):572-585.
[13]SOURAV D,ARUSHI M,SELVI B.Long non-coding RNAs,lnc(ing) RNA metabolism to cancer biology[J].Subcell Biochem,2022,100:175-199.
[14]WANG SW,LIANG K,HU QS,et al.JAK2-binding long noncoding RNA promotes breast cancer brain metastasis[J].J Clin Invest,2017,127(12):4498-4515.
[15]ZHANG M,WANG RJ,ZHAO X,et al.LncRNA LINK-a regulates ROCK1 expression in early-stage pancreatic adenocarcinoma[J].Exp Ther Med,2020,19(3):1933-1939.
[16]WANG Y,DING X,HU H,et al.Long non-coding RNA lnc-PCTST predicts prognosis through inhibiting progression of pancreatic cancer by downregulation of TACC-3[J].Int J Cancer,2018,143(12):3143-3154.
[17]GUIDALBERTO M,MONICA F.Epithelial-mesenchymal transition(EMT) 2021[J].Int J Mol Sci,2022,23(10):5848-5849.
[18]GEORGAKOPOULOS I,CHARTOUMPEKIS DV,KYRIAZOPOULOU V,et al.EMT factors and metabolic pathways in cancer[J].Front Oncol,2020,10:499-500.
[19]RIO I,KANAE M,JUN W,et al.N-cadherin localization in taste buds of mouse circumvallate papillae[J].J Comp Neurol,2021,529(9):2227-2242.
[20]ADETAYO A,ADISA A,BABATUNDE A,et al.Immunohistochemical expression of E-cadherin,N-cadherin and Snail/slug in ameloblastoma[J].J Stomatol Oral Maxillofac Surg,2022,123(6):e801-e807.
[21]CHRISTGEN M,BARTELS S,VAN JL,et al.E-cadherin to P-cadherin switching in lobular breast cancer with tubular elements[J].Mod Pathol,2020,33(12):2483-2498.
[22]ZHANG Z,LI ZL,JI HT.Direct targeting of β-catenin in the Wnt signaling pathway:Current progress and perspectives[J].Med Res Rev,2021,41(4):2109-2129.
[23]MICHAEL F,DANIIELA K,NICOLAS S,et al.Desmoglein 2 can undergo Ca2+-dependent interactions with both desmosomal and classical cadherins including E-cadherin and N-cadherin[J].Biophys J,2022,121(7):1322-1335.
[24]RABIA H,MALEEHA M,ALI H.Wnt signaling pathway:A comprehensive review[J].Cell Biol Int,2022,46(6):863-877.
[25]VINH V,TAYLOR L,BRENDAN S,et al.P120 catenin potentiates constitutive E-cadherin dimerization at the plasma membrane and regulates trans binding[J].Curr Biol,2021,31(14):3017-3027.


Last Update: 2023-05-31