|Table of Contents|

Radiosensitization of breast cancer cells and transplanted tumors by folate-modified halloysite nanotubes loaded with curcumin

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

2023 13
Research Field:
Publishing date:


Radiosensitization of breast cancer cells and transplanted tumors by folate-modified halloysite nanotubes loaded with curcumin
WANG Hanjian12HAN Dongliang2WEI Wenxiang1TIAN Weiqun1
1.Department of Biomedical Engineering,Wuhan University Taikang Medical School(School of Basic Medical Sciences),Hubei Wuhan 430071,China;2.Department of Oncology,General Hospital of Central Theater Command,Hubei Wuhan 430070,China.
halloysite nanotubescurcuminbreast cancerradiosensitization
Objective:To investigate the radiosensitizing effect of folate-modified halloysite nanotubes loaded with curcumin(HNTs-PEG-FA/Cur) on breast cancer cells MCF-7 and 4T1 transplanted tumors.Methods:The folate receptor-targeted nanomedicine HNTs-PEG-FA/Cur was prepared by chemical modification and physical adsorption,and characterized by TEM,FT-IR and XPS.The cytotoxicity of the nanodrug was detected by MTT assay,and the proapoptotic effect and in vitro radiosensitization of HNTs-PEG-FA/Cur combined with radiotherapy on MCF-7 cells were evaluated by flow cytometry,cell live/dead staining assay and cell clone formation assay.The volumetric growth inhibition of transplanted tumors was further validated by establishing 4T1 cell load-bearing mouse model.Results:HNTs were successfully modified and grafted with folic acid molecules,and curcumin was loaded into the lumen.Cytotoxicity assay showed no significant effect of HNTs-PEG-FA/Cur on cell proliferation at an equivalent curcumin concentration of 5 μmol/L.Cell live/dead staining and flow cytometry results showed that combined 6 Gy of X-ray significantly promoted apoptosis.The results of clone formation assay showed a radiosensitization ratio of 1.25 on MCF-7 cells.The results of in vivo tumor suppression assay showed that the tumor growth inhibition rate in the HNTs-PEG-FA/Cur combined with radiotherapy group(56.75%)>radiation alone group(24.16%)>drug alone group(6.67%).Conclusion:As a new nano drug carrier of curcumin,HNTs-PEG-FA can effectively improve the bioavailability of curcumin and has the potential of targeted radiosensitization for breast cancer.


[1]SUNG H,FERLAY J,SIEGEL RL,et al.Global cancer statistics 2020:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J].CA Cancer J Clin,2021,71(3):209-249.
[2]LEI S,ZHENG R,ZHANG S,et al.Breast cancer incidence and mortality in women in China:temporal trends and projections to 2030[J].Cancer Biol Med,2021,18(3):900-909.
[3]GRADISHAR WJ,ANDERSON BO,ABRAHAM J,et al.Breast cancer,version 3.2020,NCCN clinical practice guidelines in oncology[J].J Natl Compr Canc Netw,2020,18(4):452-478.
[4]LIU W,CHEN B,ZHENG H,et al.Advances of nanomedicine in radiotherapy[J].Pharmaceutics,2021,13(11):1757.
[5]SHI J,KANTOFF PW,WOOSTER R,et al.Cancer nanomedicine:progress,challenges and opportunities[J].Nat Rev Cancer,2017,17(1):20-37.
[6]XIE J,GONG L,ZHU S,et al.Emerging strategies of nanomaterial-mediated tumor radiosensitization[J].Adv Mater,2019,31(3):1802244.
[7]ZHANG P,MARILL J,DARMON A,et al.NBTXR3 radiotherapy-activated functionalized hafnium oxide nanoparticles show efficient antitumor effects across a large panel of human cancer models[J].Int J Nanomedicine,2021,12(16):2761-2773.
[8]WANG H,MU X,HE H,et al.Cancer radiosensitizers[J].Trends Pharmacol Sci,2018,39(1):24-48.
[9]SONG G,CHENG L,CHAO Y,et al.Emerging nanotechnology and advanced materials for cancer radiation therapy[J].Adv Mater,2017,29(32):1700996.
[10]侯小雪,黄帆,杨丽军,等.高分子纳米材料用于肿瘤放疗增敏的研究进展[J].国际放射医学核医学杂志,2020,44(6):381-385. HOU XX,HUANG F,YANG LJ,et al.Research progress on polymer nanomaterials for tumor radiotherapy[J].International Journal of Radiation Medicine and Nuclear Medicine,2020,44(6):381-385.
[11]陈园方,杨雨薇,唐海康,等.无机纳米材料介导的肿瘤放疗增敏的研究进展[J].国际放射医学核医学杂志,2021,45(11):710-714. CHEN YF,YANG YW,TANG HK,et al.Research progress on inorganic nanomaterial-mediated tumorradiosensitization[J].International Journal of Radiation Medicine and Nuclear Medicine,2021,45(11):710-714.
[12]王镀津,魏莉平,汪宏良.乳糖酸修饰的介孔二氧化硅纳米粒子靶向肝癌研究[J].现代肿瘤医学,2019,27(19):3403-3409. WANG DJ,WEI LP,WANG HL.Study on mesoporous silica nanoparticles modified with lactose acid targeting liver cancer[J].Modern Oncology,2019,27(19):3403-3409.
[13]HASANI M,ABDOUSS M,SHOJAEI S.Nanocontainers for drug delivery systems:A review of Halloysite nanotubes and their properties[J].Int J Artif Organs,2021,44(6):426-433.
[14]MOHAMED HI,MOHD SN,BUYS YF,et al.Recent advancement in polymer/halloysite nanotube nanocomposites for biomedical applications[J].J Biomed Mater Res B Appl Biomater,2022,110(11):2574-2588.
[15]SHEHZAD A,LEE J,LEE YS.Curcumin in various cancers[J].Biofactors,2013,39(1):56-68.
[16]GIORDANO A,TOMMONARO G.Curcumin and cancer[J].Nutrients,2019,11(10):2376.
[17]YANG G,MA R,ZHANG S,et al.Microwave-assisted in situ ring-opening polymerization of epsilon-caprolactone in the presence of modified halloysite nanotubes loaded with stannous chloride[J].RSC Adv,2022,12(3):1628-1637.
[18]YAO XH,LIU HY,XU Y,et al.Targeted and controlled release of HNTs coupling SPOP antibody loaded anticancer drugs[J].Journal of Functional Materials,2018,49(1):1121-1126.
[19]WU YP,YANG J,GAO HY,et al.Folate-conjugated halloysite nanotubes,an efficient drug carrier,deliver doxorubicin for targeted therapy of breast cancer[J].ACS Applied Nano Materials,2018,1(2):595-608.
[20]ZHU D,SHAO M,YANG J,et al.Curcumin enhances radiosensitization of nasopharyngeal carcinoma via mediating regulation of tumor stem-like cells by a circRNA network[J].Journal of Cancer,2020,11(8):2360-2370.
[21]SAK K.Radiosensitizing potential of curcumin in different cancer models[J].Nutr Cancer,2020,72(8):1276-1289.
[22]KOTHA RR,LUTHRIA DL.Curcumin:Biological,pharmaceutical,nutraceutical,and analytical aspects[J].Molecules,2019,24(16):2930.


National Natural Science Foundation of China(No.81871493);国家自然科学基金资助项目(编号:81871493)
Last Update: 2023-05-31