|Table of Contents|

Radiosensitization of breast cancer cells and transplanted tumors by folate-modified halloysite nanotubes loaded with curcumin

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 13
Page:
2379-2384
Research Field:
Publishing date:

Info

Title:
Radiosensitization of breast cancer cells and transplanted tumors by folate-modified halloysite nanotubes loaded with curcumin
Author(s):
WANG Hanjian1; 2; HAN Dongliang2; WEI Wenxiang1; TIAN Weiqun1
1.Department of Biomedical Engineering,Wuhan University Taikang Medical School(School of Basic Medical Sciences),Hubei Wuhan 430071,China;2.Department of Oncology，General Hospital of Central Theater Command，Hubei Wuhan 430070,China.
Keywords:
halloysite nanotubes; curcumin; breast cancer; radiosensitization
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2023.13.002
Abstract:
Objective:To investigate the radiosensitizing effect of folate-modified halloysite nanotubes loaded with curcumin（HNTs-PEG-FA/Cur) on breast cancer cells MCF-7 and 4T1 transplanted tumors.Methods:The folate receptor-targeted nanomedicine HNTs-PEG-FA/Cur was prepared by chemical modification and physical adsorption,and characterized by TEM,FT-IR and XPS.The cytotoxicity of the nanodrug was detected by MTT assay,and the proapoptotic effect and in vitro radiosensitization of HNTs-PEG-FA/Cur combined with radiotherapy on MCF-7 cells were evaluated by flow cytometry,cell live/dead staining assay and cell clone formation assay.The volumetric growth inhibition of transplanted tumors was further validated by establishing 4T1 cell load-bearing mouse model.Results:HNTs were successfully modified and grafted with folic acid molecules,and curcumin was loaded into the lumen.Cytotoxicity assay showed no significant effect of HNTs-PEG-FA/Cur on cell proliferation at an equivalent curcumin concentration of 5 μmol/L.Cell live/dead staining and flow cytometry results showed that combined 6 Gy of X-ray significantly promoted apoptosis.The results of clone formation assay showed a radiosensitization ratio of 1.25 on MCF-7 cells.The results of in vivo tumor suppression assay showed that the tumor growth inhibition rate in the HNTs-PEG-FA/Cur combined with radiotherapy group（56.75%)>radiation alone group（24.16%)>drug alone group（6.67%).Conclusion:As a new nano drug carrier of curcumin,HNTs-PEG-FA can effectively improve the bioavailability of curcumin and has the potential of targeted radiosensitization for breast cancer.

References:

［1］SUNG H,FERLAY J,SIEGEL RL,et al.Global cancer statistics 2020：GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries［J］.CA Cancer J Clin,2021,71(3):209-249.
［2］LEI S,ZHENG R,ZHANG S,et al.Breast cancer incidence and mortality in women in China：temporal trends and projections to 2030［J］.Cancer Biol Med,2021,18(3):900-909.
［3］GRADISHAR WJ,ANDERSON BO,ABRAHAM J,et al.Breast cancer,version 3.2020,NCCN clinical practice guidelines in oncology［J］.J Natl Compr Canc Netw,2020,18(4):452-478.
［4］LIU W,CHEN B,ZHENG H,et al.Advances of nanomedicine in radiotherapy［J］.Pharmaceutics,2021,13(11):1757.
［5］SHI J,KANTOFF PW,WOOSTER R,et al.Cancer nanomedicine：progress,challenges and opportunities［J］.Nat Rev Cancer,2017,17(1):20-37.
［6］XIE J,GONG L,ZHU S,et al.Emerging strategies of nanomaterial-mediated tumor radiosensitization［J］.Adv Mater,2019,31(3):1802244.
［7］ZHANG P,MARILL J,DARMON A,et al.NBTXR3 radiotherapy-activated functionalized hafnium oxide nanoparticles show efficient antitumor effects across a large panel of human cancer models［J］.Int J Nanomedicine,2021,12(16):2761-2773.
［8］WANG H,MU X,HE H,et al.Cancer radiosensitizers［J］.Trends Pharmacol Sci,2018,39(1):24-48.
［9］SONG G,CHENG L,CHAO Y,et al.Emerging nanotechnology and advanced materials for cancer radiation therapy［J］.Adv Mater,2017,29(32):1700996.
［10］侯小雪,黄帆,杨丽军,等.高分子纳米材料用于肿瘤放疗增敏的研究进展［J］.国际放射医学核医学杂志,2020,44(6):381-385. HOU XX,HUANG F,YANG LJ,et al.Research progress on polymer nanomaterials for tumor radiotherapy［J］.International Journal of Radiation Medicine and Nuclear Medicine,2020,44(6):381-385.
［11］陈园方,杨雨薇,唐海康,等.无机纳米材料介导的肿瘤放疗增敏的研究进展［J］.国际放射医学核医学杂志,2021,45(11):710-714. CHEN YF,YANG YW,TANG HK,et al.Research progress on inorganic nanomaterial-mediated tumorradiosensitization［J］.International Journal of Radiation Medicine and Nuclear Medicine,2021,45(11)：710-714.
［12］王镀津,魏莉平,汪宏良.乳糖酸修饰的介孔二氧化硅纳米粒子靶向肝癌研究［J］.现代肿瘤医学,2019,27(19):3403-3409. WANG DJ,WEI LP,WANG HL.Study on mesoporous silica nanoparticles modified with lactose acid targeting liver cancer［J］.Modern Oncology,2019,27(19):3403-3409.
［13］HASANI M,ABDOUSS M,SHOJAEI S.Nanocontainers for drug delivery systems：A review of Halloysite nanotubes and their properties［J］.Int J Artif Organs,2021,44(6):426-433.
［14］MOHAMED HI,MOHD SN,BUYS YF,et al.Recent advancement in polymer/halloysite nanotube nanocomposites for biomedical applications［J］.J Biomed Mater Res B Appl Biomater,2022,110(11):2574-2588.
［15］SHEHZAD A,LEE J,LEE YS.Curcumin in various cancers［J］.Biofactors,2013,39(1):56-68.
［16］GIORDANO A,TOMMONARO G.Curcumin and cancer［J］.Nutrients,2019,11(10):2376.
［17］YANG G,MA R,ZHANG S,et al.Microwave-assisted in situ ring-opening polymerization of epsilon-caprolactone in the presence of modified halloysite nanotubes loaded with stannous chloride［J］.RSC Adv,2022,12(3):1628-1637.
［18］YAO XH,LIU HY,XU Y,et al.Targeted and controlled release of HNTs coupling SPOP antibody loaded anticancer drugs［J］.Journal of Functional Materials,2018,49(1):1121-1126.
［19］WU YP,YANG J,GAO HY,et al.Folate-conjugated halloysite nanotubes,an efficient drug carrier,deliver doxorubicin for targeted therapy of breast cancer［J］.ACS Applied Nano Materials,2018,1(2):595-608.
［20］ZHU D,SHAO M,YANG J,et al.Curcumin enhances radiosensitization of nasopharyngeal carcinoma via mediating regulation of tumor stem-like cells by a circRNA network［J］.Journal of Cancer,2020,11(8):2360-2370.
［21］SAK K.Radiosensitizing potential of curcumin in different cancer models［J］.Nutr Cancer,2020,72(8):1276-1289.
［22］KOTHA RR,LUTHRIA DL.Curcumin：Biological,pharmaceutical,nutraceutical,and analytical aspects［J］.Molecules,2019,24(16):2930.

Memo

Memo:
National Natural Science Foundation of China（No.81871493）;国家自然科学基金资助项目(编号：81871493)
Last Update: 2023-05-31