|Table of Contents|

Exploring the expression of CDC20 in cervical cancer tissues based on TCGA database and its effect on cancer cell autophagy

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2025 03
Page:
375-387
Research Field:
Publishing date:

Info

Title:
Exploring the expression of CDC20 in cervical cancer tissues based on TCGA database and its effect on cancer cell autophagy
Author(s):
MO Yanxiu1; XU Ouou2; LI Liangzheng2; YU Kunlin2; HE Jiayi2; WANG Qiben1
1.School of Basic Medical Science,Xiangnan University,Hunan Chenzhou 423000,China;2.College of Clinical Studies,Xiangnan University,Hunan Chenzhou 423000,China.
Keywords:
TCGA; cervical cancer; autophagy; prognosis; CDC20
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2025.03.004
Abstract:
Objective:To explore the expression of cell division cycle protein 20 (CDC20) in cervical cancer (CC) tissues and analyzing the effect of CDC20 on autophagy in cervical cancer cells.Methods:The TCGA data were used to analyze CDC20 expression in CC tissues and the effects of CDC20 expression on survival ratio and clinical stage of cervical cancer patients.The correlation of CDC20 with autophagy pathway was analyzed by GSEA enrichment analysis.The protein-protein interaction (PPI) network of CDC20 was anaylyzed using STRING database,and immune cell infiltration was analyzed by ssGSEA and Spearman's analysis.CDC20 expression in cervical carcinoma and adjacent tissues were detected by immunohistochemistry method.The sgRNA2&7-CDC20 dual-gene knockout stable cell strain was constructed by CRISPR-cas9 method.The experiments were divided into sgRNA-NC,sgRNA-CDC20,sgRNA-NC+serum-starved for 24 h group and sgRNA-CDC20+serum-starved for 24 h group.Changes in autophagosomes in cells were detected by transmission electron microscopy (TEM).The subcellular localization,mRNA,and protein expression levels of the autophagy genes p62,Beclin 1,and LC3 were determined by immunofluorescence,qRT-PCR,and Western Blot analysis,respectively.Results:CDC20 was highly expressed in cervical cancer tissues compared to adjacent tissues,which was highly correlated with patient age,clinical stage,immune infiltration,and poor prognosis (P＜0.05),and shorter overall survival (P＜0.001).GSEA analysis suggested that high expression of CDC20 participates in nine pathways including autophagy pathway,and the STRING database analysis suggested that 53 proteins could interact with CDC20.Immunohistochemistry results showed that CDC20 expression was significantly higher in cervical cancer tissues than in adjacent normal tissues.TEM results showed that CDC20 knockdown induced autophagy in cervical cancer C33A cells.Immunofluorescence results showed that p62,Beclin 1 and LC3 were mainly localized in the cytoplasm,and the knockdown of CDC20 or serum starvation for 24 h result in significant increased fluorescence intensity of LC3 and the two conditions showed superimposed effect.qRT-PCR and Western Blot analysis revealed that the knockout of CDC20 resulted in increased formation of acidic autophagosome vesicles and significantly enhanced the expression of autophagy-related protein LC3B-II/I(P＜0.05).Conclusion:CDC20 is highly expressed in cervical cancer tissues and cells and affects autophagy.CDC20 may be an independent prognostic marker for CC and a new target for molecular targeted therapy.

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Memo

Memo:
湖南省教育厅科学研究项目(湘教通［2023］361号，编号:23A0589)；湖南省大学生创新训练计划项目(湘教通［2024］191号，编号:4854);湘南学院2020高层次人才科研启动基金(编号:2021A20)
Last Update: 1900-01-01