|Table of Contents|

The effect of miR-19a regulating TGF-β1/Smad signal pathway on the proliferation,invasion and glycolysis of gastric cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 22
Page:
4278-4284
Research Field:
Publishing date:

Info

Title:
The effect of miR-19a regulating TGF-β1/Smad signal pathway on the proliferation,invasion and glycolysis of gastric cancer cells
Author(s):
SHAN Biao1; BIAN Liang2; LI Shujun2; WANG Peixian2; WU Dianchao3; LEI Qiuxiang1; LIU Dengxiang4
1.Department of Blood Transfusion;2.Department of Laboratory Medicine;3.Department of Gastrointestinal Oncology;4.Central Laboratory,Xingtai People's Hospital,Hebei Xingtai 054001,China.
Keywords:
gastric cancer; miRNA-19a; transforming growth factor-β1/Smad; proliferation; invasion; glycolysis
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2024.22.010
Abstract:
Objective:To investigate the effects of microRNA-19a (miR-19a) on the proliferation,invasion and glycolysis of human gastric cancer AGS cells by regulating transforming growth factors-β1(TGF-β1)/Smad signal pathway.Methods:Human gastric cancer AGS cells were cultured in vitro and divided into control groups.Liposomes were used for transfection.Inhibitor NC,mimics NC,miR-19a inhibitor,miR-19a mimics were transfected into human gastric cancer AGS cells and recorded as inhibitor NC group,mimics NC group,miR-19a inhibitor group and miR-19a mimics group.The expression of miR-19a was detected by real-time fluorescent quantitative PCR (RT-qPCR),and the cell viability was detected by cell counting kit-8 (CCK-8).It was found that knockdown of miR-19a could significantly inhibit the activity of gastric cancer AGS cells.Therefore,the follow-up experiment was divided into control group,inhibitor NC group,miR-19a inhibitor group and inhibitor group (miR-19a inhibitor transfection+10 μmol/L TGF-β1/Smad pathway inhibitor LY2109761),activator group (miR-19a inhibitor transfection+10 μmol/L TGF-β1/Smad pathway activator SRI-011381).The miR-19a expression level,proliferation rate,invasion number,glucose consumption,lactic acid level,glycolysis,and expression level of related proteins were analyzed by real-time fluorescent quantitative PCR (RT-qPCR),5-acetyl-2 'deoxyuridine (EdU),Transwell chamber,lactic acid,glucose detection kit,and western blot (WB).Results:It was found that knockdown of miR-19a could significantly inhibit the viability of gastric cancer AGS cells,so miR-19a inhibitor was transfected for subsequent pathway validation experiments.The results showed that the expression level of miR-19a,cell proliferation rate,invasion rate,glucose consumption and lactic acid level,PCNA,HK2,LDHA,GAPDH,TGF-β1,p-Smad3 protein expression level was no significant difference in control group and inhibitor NC group(P＞0.05).The expression of miR-19a,proliferation rate,invasion number,glucose consumption,lactate level,PCNA,HK2,LDHA,GAPDH,TGF-β1,p-Smad3 protein levels of miR-19a inhibitor group were lower than those of miR-19a inhibitor group (P＜0.05).The expression,proliferation rate,invasion number,glucose consumption,lactate level,PCNA,HK2,LDHA,GAPDH,TGF-β1 and p-Smad3 protein expression levels of miR-19a inhibitor group were lower than those of miR-19a inhibitor group (P＜0.05).Conclusion:Down regulating the expression of miR-19a can inhibit the proliferation,invasion and glycolysis of human gastric cancer AGS cells,and its mechanism of action is to block TGF-β1/Smad signal transduction.

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2023年度河北省医学科学研究课题计划（编号：20232013）
Last Update: 1900-01-01