|Table of Contents|

Construction of NMU gene knockout mouse pancreatic orthotopic tumor model and its significance

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 22
Page:
4273-4277
Research Field:
Publishing date:

Info

Title:
Construction of NMU gene knockout mouse pancreatic orthotopic tumor model and its significance
Author(s):
ZHENG Rui; PAN Xuyi; GONG Zixuan; GUO Li
School of Basic Medical Science,Shenyang Medical College,Liaoning Shenyang 110034,China.
Keywords:
NMU; genotype identification; pancreatic tumor bearing in situ; bioinformatics analysis; flow cytometry
PACS:
R735.9
DOI:
10.3969/j.issn.1672-4992.2024.22.009
Abstract:
Objective:NMU gene knockout C57BL/6 mice were used for establishing orthotopic pancreatic cancer models.It provides a prerequisite foundation for studying the molecular mechanisms of pancreatic cancer development and finding new immunotherapy strategies.Methods：According to the NMU gene sequence structure,knockout identification primers were designed within the second exon,and mouse genotypes were identified using PCR and agarose gel electrophoresis.Heterozygous mice were propagated in a cage with male and female in a ratio of 2∶1,in order to obtain NMU gene knockout homozygous mice.2×107 Panc02 cells were inoculated into the pancreatic tail of homozygous NMU gene knockout mice to establish an orthotopic tumor-bearing model.Bioinformatics analysis of the differential expression of NMU,patient survival period,clinical pathological analysis and changes in the tumor immune microenvironment.Flow cytometry analysis of CD8+T cell infiltration in the spleen of tumor-bearing mice after NMU knockout.Results：After breeding and identification of C57BL/6 NMU gene knockout heterozygous mice,NMU gene knockout homozygous mice were obtained,and the pancreatic tail was inoculated with Panc02 cells to successfully construct a pancreatic orthotopic tumor model.Bioinformatics analysis shows that NMU,as an early and continuous event in the development of pancreatic cancer,causes poor prognosis of patients and makes the tumor microenvironment in an immunosuppressive state.Flow cytometry showed that the expression of CD8+T cells increased in the systemic immune landscape of tumor-bearing mice after NMU knockout.Conclusion：The C57BL/6 NMU gene knockout mice were established to construct an in situ tumor bearing model of the pancreas,which provides a prerequisite basis for the study of NMU as a prognostic adverse event in the occurrence and development of pancreatic cancer and the study of immunosuppression mechanism of pancreatic cancer.

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沈阳医学院大学生科研项目（编号：20249080）
Last Update: 1900-01-01