|Table of Contents|

miR-335-5p regulates TGF-β/Smad signaling pathway to inhibit EMT,invasion and migration of gastric cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 22
Page:
4228-4234
Research Field:
Publishing date:

Info

Title:
miR-335-5p regulates TGF-β/Smad signaling pathway to inhibit EMT,invasion and migration of gastric cancer cells
Author(s):
NING Yue1; 2; 3; CHEN Fengqin1; ZHAO Xueling1; SHAO Lihua2; 3; WANG Meng2; 3; WANG Hongwei1; LI Hailong1; 2; 3
1.Affiliated Hospital of Gansu University of Traditional Chinese Medicine,Gansu Lanzhou 730000,China;2.Department of Internal Medicine,the First Clinical Medical College of Gansu University of Traditional Chinese Medicine,Gansu Lanzhou 730000,China;3.Key Laboratory of Traditional Chinese Herbs and Prescription Innovation and Transformation of Gansu Province,Gansu Lanzhou 730000,China.
Keywords:
miR-335-5p; TGF-β/Smad; gastric cancer; EMT
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2024.22.003
Abstract:
Objective:To investigate the effects of upregulation of microRNA (miR)-335-5p on the invasion,migration and the expressions of EMT markers in gastric cancer cells via TGF-β/Smad signaling pathway.Methods:Gastric cancer cells were infected with miR-335-5p lentiviral vector,and screened by puromycin to establish a stable cell line.The effects of up-regulated miR-335-5p on the invasion and migration of gastric cancer cells were observed by scratch and Transwell experiments.The effects of up-regulation of miR-335-5p on mRNA and protein expression levels of key genes of TGF-β signaling pathway and the genes related to epithelial interstitial transition (EMT) in gastric cancer cells were detected by RT-qPCR and Western-blot assay.Results:The scratch results showed that compared with the negative control group,the healing rate of the two gastric cancer cells was significantly decreased after up-regulation of miR-335-5p (P＜0.05,P＜0.01).The results of Transwell migration and invasion experiments showed that the number of cells passing through the chamber of the two gastric cancer cell lines in the miR-335-5p overexpression group was significantly lower than that in the negative control group (P＜0.05,P＜0.01).The experiments results showed that the expressions of TGF-β1,TGF-β2,TGF-βR2,p-Smad2 and p-Smad3 and Smad4 protein in miR-335-5p upregulation group were significantly lower than the negative control group (P＜0.05,P＜0.01).The expressions of a-SmA,Collagen1,N-cadherin,Cytokinetin18,Claudin7,ZO-1,Vimentin,Snail1,Snail2,FN1,Twist2 mRNA and protein in the miR-335-5p overexpression group was significantly lower than the negative control group (P＜0.05,P＜0.01),while E-cadherin mRNA and protein levels higher than the negative control group (P＜0.05,P＜0.01).Conclusion:Overexpression of miR-335-5p regulates EMT via TGF-β/Smad signaling pathway,thereby inhibiting the invasion and migration of gastric cancer cells.

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Memo

Memo:
甘肃省卫生行业计划项目（编号：GSWSKY2022-25）;甘肃中医药大学附属医院院内创新基金项目（编号：gzfy-2022-12）
Last Update: 1900-01-01