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LncRNA DLEU2 regulates the proliferation and apoptosis of papillary thyroid carcinoma cells through autophagy mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 21
Page:
4043-4049
Research Field:
Publishing date:

Info

Title:
LncRNA DLEU2 regulates the proliferation and apoptosis of papillary thyroid carcinoma cells through autophagy mechanism
Author(s):
ZHAO Ruhua1; HUO Ronghua1; LIN Xu1; ZHANG He1; LI Min1; 2; WU Jingfang1; XUE Gang1; 2
1.Key Laboratory of Zhangjiakou City，Hebei Zhangjiakou 075000,China;2.Department of Otolaryngology Head and Neck Surgery,the First Affiliated Hospital of Hebei North University,Hebei Zhangjiakou 075000,China.
Keywords:
DLEU2; papillary thyroid carcinoma; autophagy; cell proliferation; apoptosis
PACS:
R736.1
DOI:
10.3969/j.issn.1672-4992.2024.21.005
Abstract:
Objective:To explore whether LncRNA DLEU2 affects the proliferation and apoptosis of thyroid papillary carcinoma cells through autophagy.Methods:Detecting expression of Beclin-1 and ATG7 in 17 pairs of papillary thyroid carcinomas and paracancerous tissues by immunohistochemistry and quantitative real-time PCR (qRT-PCR).DLEU2 expression in TPC-1 cells and normal thyroid cells were detected by qRT-PCR.The DLEU2 silencing vector (shDLEU2) and control vector (shNC) were transfected into the thyroid papillary carcinoma TPC-1 cell line by LipofectamineTM3000 transfection reagent.The experiment was divided into a blank control group (non transfected TPC-1 cells),a DLEU2 silencing group (shDLEU2 transfected TPC-1 cells),and a negative control groups(shCN transfected TPC-1 cells).Using growth curves,scratch experiments,and Transwell to detect the proliferation,migration,and invasion abilities of TPC-1 cells.Flow cytometry was used to detect the effect of silencing DLEU2 on the apoptosis of TPC-1.Western blot and immunocytochemistry were used to detect the effect of DLEU2 on the expression of Beclin-1,ATG7,and LC3 in TPC-1 cells.Results:IHC results showed that ATG7 and Beclin-1 were highly expressed in papillary thyroid carcinoma (P＜0.05).In silencing DLEU2 group,the proliferation,migration,and invasion ability of TPC-1 cells decreased (P＜0.05).Western blot showed silencing of DLEU2 gene,increased expression of Beclin-1 and ATG7,and decreased LC3 A/B ratio (P＜0.05).Conclusion:Silencing the DLEU2 gene may promote apoptosis of TPC-1 and inhibit its proliferation,migration and invasion through autophagy.

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Last Update: 2024-09-30