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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2024 21

Page:

4035-4043

Research Field:

Publishing date:

Info

Title:

LINC01419 promotes the proliferation,migration and invasion of liver cancer cells by activating the replication of hepatitis B virus

Author(s):

WAN Meiling¹; LI Decheng¹; SUN Changfeng¹; LIU Xiaoling¹; SHENG Yunjian¹; LI Yaling²; DENG Cunliang¹

1.Department of Infectious Diseases,the Affiliated Hospital of Southwest Medical University,Laboratory of Infection and Immunology,Sichuan Luzhou 646000,China;2.Department of Pharmacy,the Affiliated Hospital of Southwest Medical University,Sichuan Luzhou 646000,China.

Keywords:

hepatocellular carcinoma; LINC01419; hepatitis B virus; proliferation; metastasis

PACS:

R735.7

DOI:

10.3969/j.issn.1672-4992.2024.21.004

Abstract:

Objective:To explore the function and mechanism of LINC01419 in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC).Methods:Analysis of HCC patient data from the TCGA database to determine the clinical value of LINC01419 in patients with HBV-related HCC.The expression level of LINC01419 in HCC cells was assessed using real-time quantitative reverse PCR(qRT-PCR).The impact of HBx protein on LINC01419 expression and the effects of LINC01419 on HBV indicator markers (HBx,pgRNA,cccDNA) were investigated.Various assays including CCK-8,Transwell,cell scratch,and flow cytometry were employed to assess the effects of LINC01419 on cell proliferation,migration and invasion in HCC cells.Results:LINC01419 had a significant effect on the survival of patients with HBV-associated HCC.The expression of LINC01419 was higher in HBV-positive HepG2.215 cells than in HBV-negative HepG2 cells.Moreover,in HBV-producing HepAD38 cells and HepG2-NTCP cells after infection with HBV,LINC01419 expression levels were significantly increased.Overexpression of HBx protein in cells significantly increased the expression of LINC01419.Increasing the level of LINC01419 increased the levels of HBV-associated markers HBx,pgRNA,and cccDNA in HepG2.215 cells.Overexpression of LINC01419 promoted the proliferation,migration,and invasion of HCC cells,and the promotion was more pronounced in HepG2.215 cells and HepAD38£¨HBV-positive£© cells than in HepG2 and HepAD38£¨HBV-negative£© cells.Conclusion:LINC01419 activates HBV replication,while LINC01419 and HBV synergistically promote the malignant progression of HBV-associated HCC.

References:

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Last Update: 2024-09-30