|Table of Contents|

Baicalin inhibits the malignant progression of pancreatic cancer cells and its related mechanisms

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 21
Page:
4015-4022
Research Field:
Publishing date:

Info

Title:
Baicalin inhibits the malignant progression of pancreatic cancer cells and its related mechanisms
Author(s):
WEI Bian1; REN Shuai1; TIAN Ying1; SONG Lina1; QIAN Lichao2; WANG Zhongqiu1
1.Affiliated Hospital of Nanjing University of Chinese Medicine(Jiangsu Provincial Hospital of Traditional Chinese Medicine),Jiangsu Nanjing 210029,China;2.Department of Geriatrics,Nanjing Hospital of Chinese Medicine,Jiangsu Nanjing 210022,China.
Keywords:
pancreatic cancer; baicalin; cell proliferation; migration and invasion; apoptosis
PACS:
R735.9
DOI:
10.3969/j.issn.1672-4992.2024.21.001
Abstract:
Objective:To compare the therapeutic effects of baicalin on various pancreatic cancer cells to investigate its anti-tumor effects on pancreatic cancer in vitro.Methods:Human pancreatic ductal epithelial cells (hTERT-HPNE) and pancreatic cancer cell lines (PANC-1,MIA PaCa-2 and BxPC-3) were cultured and treated with different concentrations (0,25,50,100,200 μmol/L) of baicalin.CCK-8 assay,Calcein AM/PI Staining and clone formation assay were performed to detect the effect of baicalin on proliferation in pancreatic cancer cells.Cell migration and invasion were assessed with wound healing assay and Transwell invasion assay.Flow cytometry analysis was performed to detect the effect of baicalin on apoptosis in pancreatic cancer cells，and Western blot analysis was performed to examine the expression of BCL-2,BAX,Caspase3.Results:Baicalin had varying degrees inhibition on the proliferation,migration,invasion and promoted apoptosis of the pancreatic cancer cells (PANC-1,MIA PaCa-2 and BxPC-3).It is also possible to induce potential apoptosis by up-regulating the protein level of Caspase3 and the ratio of BAX/BCL-2,while has no obvious effect on pancreatic ductal epithelial cells (hTERT-HPNE).Conclusion:Our work demonstrated that baicalin exhibited good biological safety and low toxicity to normal pancreatic cell,but it has significant anti-cancer properties and is expected to be a potential drug for the treatment of pancreatic cancer.

References:

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Memo

Memo:
National Natural Science Foundation of China（No.82202135,82371919,82372017,82171925,81971681）;国家自然科学基金（编号：82202135,82371919,82372017,82171925,81971681）；江苏省重点研发计划社会发展项目（编号：BE2023789）；中国博士后科学基金面上项目（编号：2023M741808）；江苏省科协青年科技人才托举工程（编号：JSTJ-2023-WJ027）；南京中医药大学自然科学基金（编号：XZR2023036,XZR2021003,XZR2021050）；江苏省中医院优秀青年博士培养专项基金（编号：2023QB0112）；国家中医临床研究基地开放课题（编号：JD2023SZ16）；江苏省中医院高峰人才项目（编号：y2021rc03,y2021rc44）；江苏省研究生实践创新计划项目（编号：SJCX24_0912）
Last Update: 2024-09-30