|Table of Contents|

Effect of galangin on malignant biological behavior of pancreatic cancer cells by regulating Notch-1/Hes signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 20
Page:
3850-3856
Research Field:
Publishing date:

Info

Title:
Effect of galangin on malignant biological behavior of pancreatic cancer cells by regulating Notch-1/Hes signaling pathway
Author(s):
QIU Lujie1; LIAO Dazhong1; DENG Feng1; ZHAO Tianzhu2
1.Second Department of Oncology and Hematology,the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University,Sichuan Luzhou 620000,China;2.Respiratory and Critical Care Medical Center,Meishan Traditional Chinese Medicine Hospital,Sichuan Meishan 620010,China.
Keywords:
galangin; Notch-1/Hes signaling pathway; pancreatic cancer; proliferation; migration; invasion
PACS:
R735.9
DOI:
10.3969/j.issn.1672-4992.2024.20.006
Abstract:
Objective:To investigate the effect of galangin (Gal) on the malignant biological behavior of pancreatic cancer cells by regulating Notch-1/Hes signaling pathway.Methods:Human pancreatic cancer PCNA-1 cells were grouped into blank group,Gal low-dose group,Gal medium-dose group,Gal high-dose group,Jagged1 group,and Gal high-dose+Jagged1 group.EdU staining,CCK-8,scratch assay,and Transwell assay were applied to detect the proliferation,migration,and invasion of PCNA-1 cells,respectively.qRT-PCR was applied to detect the expression of Cyclin D1,matrix metalloproteinase-9,and MMP-2 mRNA in PCNA-1 cells.Nude mouse in vivo tumor transplantation experiment was applied to detect the mass and volume of tumors in nude mice.Western blot was applied to detect the expression of Notch-1 and Hes1 proteins in PCNA-1 cells and tumor tissues.Results:Compared with the blank group,the EdU positive cell rate,OD450 value,scratch healing rate,cell invasion number,Cyclin D1,MMP-9,MMP-2 mRNA expression,Notch-1,Hes1 protein expression in PCNA-1 cells were reduced in the Gal low-dose group,Gal medium-dose group,and Gal high-dose group,and were dose-dependent,the change trend of corresponding indicators in Jagged1 group was opposite to the above (P＜0.05).Compared with the Gal high-dose group,the EdU positive cell rate,OD450 value,scratch healing rate,number of cell invasions,Cyclin D1,MMP-9,MMP-2 mRNA expression,and Notch-1 and Hes1 protein expression in PCNA-1 cells in Gal high-dose+Jagged1 group increased (P＜0.05).Compared with the nude mice-blank group,the tumor mass and volume,and the expression of Notch-1 and Hes1 proteins in the nude mouse Gal low-dose group,the nude mouse Gal medium-dose group,and the nude mouse Gal high-dose group were reduced,in a dose-dependent manner (P＜0.05),the change trends of corresponding indicators in the nude mice-Jagged1 group were opposite to the above (P＜0.05).Compared with the nude mice high-dose group,the tumor mass and volume,and the expression of Notch-1 and Hes1 proteins in the nude mice Gal high-dose+Jagged1 group increased (P＜0.05).Conclusion:Gal inhibits the proliferation,migration,invasion,and tumor growth of PCNA-1 cells in nude mice,which may be related to the inhibition of the Notch-1/Hes pathway.

References:

［1］ SIEGEL RL,MILLER KD,WAGLE NS,et al.Cancer statistics,2023［J］.CA Cancer J Clin,2023,73(1):17-48.
［2］SUNG H,FERLAY J,SIEGEL RL,et al.Global cancer statistics 2020:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries［J］.CA Cancer J Clin,2021,71(3):209-249.
［3］ SPRINGFELD C,JGER D,BCHLER MW,et al.Chemotherapy for pancreatic cancer［J］.Presse Med,2019,48(2):159-174.
［4］ LEE S,HONG E,JO E,et al.Gossypol induces apoptosis of human pancreatic cancer cells via CHOP/Endoplasmic reticulum stress signaling pathway［J］.J Microbiol Biotechnol,2022,32(5):645-656.
［5］TULI HS,SAK K,ADHIKARY S,et al.Galangin:A metabolite that suppresses anti-neoplastic activities through modulation of oncogenic targets［J］.Exp Biol Med (Maywood),2022,247(4):345-359.
［6］LIU F,XIANG Q,LUO Y,et al.A hybrid nanopharmaceutical for specific-amplifying oxidative stress to initiate a cascade of catalytic therapy for pancreatic cancer［J］.J Nanobiotechnology,2023,21(1):165-185.
［7］SUBRAMANIAM D,NICHOLES ND,DHAR A,et al.3,5-bis(2,4-difluorobenzylidene)-4-piperidone,a novel compound that affects pancreatic cancer growth and angiogenesis［J］.Mol Cancer Ther,2011,10(11):2146-2156.
［8］卢丹,彭小兰,熊珊,等.高良姜素促进胰腺癌PCNA-1细胞凋亡和自噬并抑制移植瘤生长［J］.广州中医药大学学报,2021,38(9):1963-1971. LU D,PENG XL,XIONG S,et al.Galangin promotes apoptosis and autophagy of pancreatic cancer PCNA-1 cells and inhibits the growth of transplanted tumors ［J］.Journal of Guangzhou University of Traditional Chinese Medicine,2021,38(9):1963-1971.
［9］张淑艳,张方,姬颖华.鼠李素下调Notch-1的表达对乳腺癌MCF-7细胞增殖、侵袭和迁移的调节作用［J］.中国临床解剖学杂志,2018,36(6):632-636. ZHANG SY,ZHANG F,JI YH.The regulatory effect of murine down-regulation of Notch-1 expression on the proliferation,invasion and migration of breast cancer MCF-7 cells ［J］.Chinese Journal of Clinical Anatomy,2018,36(6):632-636.
［10］ZHAO X,TANG Z,YUE C,et al.Hesperidin regulates Jagged1/Notch1 pathway to promote macrophage polarization and alleviate lung injury in mice with bronchiolitis［J］.Acta Academiae Medicinae Sinicae,2022,44(5):777-784.
［11］YANG L,BAI Y,LI Q,et al.Analysis of the curative effect of neoadjuvant therapy on pancreatic cancer［J］.Front Oncol,2021,11(1):695645-695653.
［12］QADDOORI MH,AL-SHMGANI HS.Galangin-loaded gold nanoparticles:molecular mechanisms of antiangiogenesis properties in breast cancer［J］.Int J Breast Cancer,2023,2023(1):3251211-3251224.
［13］LIANG X,WANG P,YANG C,et al.Galangin inhibits gastric cancer growth through enhancing STAT3 mediated ros production［J］.Front Pharmacol,2021,12(1):646628-646640.
［14］KWAK MK,YANG KM,PARK J,et al.Galangin enhances TGF-β1-mediated growth inhibition by suppressing phosphorylation of threonine 179 residue in Smad3 linker region［J］.Biochem Biophys Res Commun,2017,494(3-4):706-713.
［15］ATWA G,OMRAN G,ELBAKY AA,et al.The antitumour effect of galangin and luteolin with doxorubicin on chemically induced hepatocellular carcinoma in rats［J］.Contemp Oncol (Pozn),2021,25(3):174-184.
［16］KIM YJ,JUNG CJ,NA H,et al.Cyclin D1 and PRAME expression in distinguishing melanoma in situ from benign melanocytic proliferation of the nail unit［J］.Diagn Pathol,2022,17(1):41-50.
［17］CUI Q,WANG X,ZHANG Y,et al.Macrophage-derived MMP-9 and MMP-2 are closely related to the rupture of the fibrous capsule of hepatocellular carcinoma leading to tumor invasion［J］.Biol Proced Online,2023,25(1):8-20.
［18］WANG Y,CHANG J,WANG ZQ,et al.Sirt3 promotes the autophagy of HK-2 human proximal tubular epithelial cells via the inhibition of Notch-1/Hes-1 signaling［J］.Mol Med Rep,2021,24(3):634-642.
［19］朱雷,盛伟伟,董明,等.Notch1在胰腺癌中的表达及临床病理学意义［J］.中国医科大学学报,2014,43(4):320-323. ZHU L,SHENG WW,DONG M,et al.Notch1 expression in pancreatic cancer and its clinicopathological significance ［J］.Journal of China Medical University,2014,43(4):320-323.
［20］LIAN H,HUI Y,XIAOPING T,et al.Baicalein suppresses the proliferation of human cervical cancer cells via Notch 1/Hes signaling pathway［J］.J Cancer Res Ther,2019,15(6):1216-1220.

Memo

Memo:
四川省南充市科技计划项目（编号：21YFZJ0063）
Last Update: 1900-01-01