|Table of Contents|

miR-133a-5p inhibits cervical cancer cell proliferation,migration,and invasion by regulating pyroptosis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 20
Page:
3840-3846
Research Field:
Publishing date:

Info

Title:
miR-133a-5p inhibits cervical cancer cell proliferation,migration,and invasion by regulating pyroptosis
Author(s):
HU Ping1; PENG Wei2; WANG Wenhua1
1.Department of Gynecology;2.Department of Ultrasound,Pingxiang People's Hospital,Jiangxi Pingxiang 337000,China.
Keywords:
miR-133a-5p; caspase-3; GSDME; cell pyroptosis; cervical cancer
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2024.20.004
Abstract:
Objective:To investigate the mechanism of miR-133a-5p targeting caspase-3 to regulate pyroptosis on the proliferation,migration and invasion of cervical cancer cells.Methods:Hela cells were divided into three groups:Control group (normal cultured cervical cancer Hela cells),mimic-NC group (cervical cancer Hela cells transfected with mimic-NC) and miR-133a-5p mimic group (cervical cancer Hela cells transfected with miR-133a-5p mimic overexpression).CCK-8 was used to detect cell proliferation,staining was used to detect cell migration and invasion,ELISA was used to detect the level of reactive oxygen species (ROS),flow cytometry was used to detect the level of apoptosis,Western Blot was used to detect the expression of caspase-3,cleaved caspase-3,GSDME and GSDME-NT,real-time fluorescence quantitative polymerase chain reaction was used to detect the mRNA expression of caspase-3 and miR-133a-5p.Results:There was no significant difference in the results of cell proliferation,migration and invasion between the control group and mimic-NC group,but the results of cell proliferation,migration and invasion in miR-133a-5p mimic group were significantly inhibited compared with those in mimic-NC group (P＜0.05).Flow cytometry showed that the level of apoptosis in miR-133a-5p mimic group was significantly higher than that in mimic-NC group (P＜0.05).The results of ELISA detection showed that the level of ROS in miR-133a-5p mimic group was significantly higher than that in mimic-NC group (P＜0.05),and the results of Western Blot and PCR showed that overexpression of miR-133a-5p could significantly increase the expression of cleaved caspase-3 and GSDME-NT and decrease the expression of caspase-3 and GSDME compared with mimic-NC group (P＜0.05).Conclusion:The inhibitory effect of miR-133a-5p on the proliferation,migration and invasion of Hela cells may be related to the targeted regulation of caspase-3 to regulate the pyroptosis of Hela cells.The intervention of caspase-3/GSDME pathway is expected to become a new direction of cervical cancer therapy.

References:

［1］ SUNG H,FERLAY J,SIEGEL RL,et al.Global cancer statistics 2020:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries ［J］.CA:A Cancer Journal for Clinicians,2021,71(3):209-249.
［2］ FERRALL L,LIN KY,RODEN RBS,et al.Cervical cancer immunotherapy:Facts and hopes ［J］.Clinical Cancer Research,2021,27(18):4953-4973.
［3］ Standardized Management and Practice Branch of Immunization Program of China Vaccine Industry Association.Expert consensus on human papillomavirus vaccine immunization strategy and practice for adolescent women in the Yangtze River Delta ［J］.Chinese Journal of Preventive Medicine,2023,57(2):135-153.
［4］ LI K,QIU J,PAN J,et al.Pyroptosis and its role in cervical cancer ［J］.Cancers,2022,14(23):5764.
［5］ YAO F,JIN Z,ZHENG Z,et al.HDAC11 promotes both NLRP3/caspase-1/GSDMD and caspase-3/GSDME pathways causing pyroptosis via ERG in vascular endothelial cells ［J］.Cell Death Discov,2022,8(1):112.
［6］ DIENER C,KELLER A,MEESE E.Emerging concepts of miRNA therapeutics:from cells to clinic ［J］.Trends in Genetics,2022,38(6):613-626.
［7］ YANG X,CHEN D,HU J,et al.miR-133a-5p inhibits glioma cell proliferation by regulating IGFBP3［J］.Journal of Oncology,2022,2022:8697676.
［8］ YUAN LY,ZHOU M,LV H,et al.Involvement of NEAT1/miR-133a axis in promoting cervical cancer progression via targeting SOX4［J］.J Cell Physiol,2019,234(10):18985-18993.
［9］ MAYADEV JS,KE G,MAHANTSHETTY U,et al.Global challenges of radiotherapy for the treatment of locally advanced cervical cancer ［J］.Int J Gynecol Cancer,2022,32(3):436-445.
［10］ BRUNI L,SERRANO B,ROURA E,et al.Cervical cancer screening programmes and age-specific coverage estimates for 202 countries and territories worldwide:a review and synthetic analysis ［J］.Lancet Glob Health,2022,10(8):e1115-e1127.
［11］ CASERTA S,GANGEMI S,MURDACA G,et al.Gender differences and miRNAs expression in cancer:Implications on prognosis and susceptibility ［J］.Int J Mol Sci,2023,24(14):11544.
［12］ ZHOU X,XIA LM.Research on the role of microRNAs in cervical cancer［J］.Modern Oncology,2019,27(17):3164-3166.
［13］ WANG SN,LYU J,ZHANG QY,et al.miRNA and invasion and metastasis of cancer［J］.Modern Oncology,2016,24（03）：485-487.
［14］ SIU MC,VOISEY J,ZANG T,et al.MicroRNAs involved in human skin burns,wound healing and scarring ［J］.Wound Repair Regen,2023,31(4):439-453.
［15］ HAN YH,MA DY,LEE SJ,et al.Bioinformatics analysis of novel targets for treating cervical cancer by immunotherapy based on immune escape ［J］.Cancer Genomics Proteomics,2023,20(4):383-397.
［16］ XIN HL,NIU SQ,LI XQ,et al.The methylation status of microRNA-449a and its expression in cervical cancer tissues［J］.Modern Oncology,2022,30（18）：3363-3366.
［17］ LI C,LI X,GAO S,et al.MicroRNA-133a inhibits proliferation of gastric cancer cells by downregulating ERBB2 expression ［J］.Oncology Research,2017,25(7):1169-1176.
［18］ CUI W,ZHANG S,SHAN C,et al.microRNA-133a regulates the cell cycle and proliferation of breast cancer cells by targeting epidermal growth factor receptor through the EGFR/Akt signaling pathway ［J］.The FEBS Journal,2013,280(16):3962-3974.
［19］ KOJIMA S,CHIYOMARU T,KAWAKAMI K,et al.Tumour suppressors miR-1 and miR-133a target the oncogenic function of purine nucleoside phosphorylase (PNP) in prostate cancer ［J］.British Journal of Cancer,2012,106(2):405-413.
［20］ VASUDEVAN SO,BEHL B,RATHINAM VA.Pyroptosis-induced inflammation and tissue damage ［J］.Semin Immunol,2023,69:101781.
［21］ LOVELESS R,BLOOMQUIST R,TENG Y.Pyroptosis at the forefront of anticancer immunity ［J］.Journal of Experimental & Clinical Cancer Research,2021,40(1):264.
［22］ SHAO F.Gasdermins:making pores for pyroptosis ［J］.Nature Reviews Immunology,2021,21(10):620-621.
［23］ DING J,WANG K,LIU W,et al.Pore-forming activity and structural autoinhibition of the gasdermin family ［J］.Nature,2016,535(7610):111-116.
［24］ ZHANG F,LIU Q,GANESAN K,et al.The antitriple negative breast cancer efficacy of Spatholobus suberectus Dunn on ROS-induced noncanonical inflammasome pyroptotic pathway ［J］.Oxidative Medicine and Cellular Longevity,2021,2021:5187569.
［25］ GANESAN K,SUKALINGAM K,XU B.Impact of consumption of repeatedly heated cooking oils on the incidence of various cancers-A critical review ［J］.Critical Reviews in Food Science and Nutrition,2019,59(3):488-505.
［26］ GANESAN K,XU B.Molecular targets of vitexin and isovitexin in cancer therapy:a critical review ［J］.Annals of the New York Academy of Sciences,2017,1401(1):102-113.
［27］ TENG JF,MEI QB,ZHOU XG,et al.Polyphyllin VI induces Caspase-1-mediated pyroptosis via the induction of ROS/NF-κB/NLRP3/GSDMD signal axis in non-small cell lung cancer ［J］.Cancers,2020,12(1):193.

Memo

Memo:
2024年度江西省卫生健康委科技计划项目（编号：202410755）
Last Update: 1900-01-01