|Table of Contents|

The serum exosomes of patients with esophageal cancer promote the proliferation and migration of cancer cells through Wnt/β-catenin and PI3K-AKT signaling pathways

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 20
Page:
3823-3828
Research Field:
Publishing date:

Info

Title:
The serum exosomes of patients with esophageal cancer promote the proliferation and migration of cancer cells through Wnt/β-catenin and PI3K-AKT signaling pathways
Author(s):
MA Lili1; LI Zimu2; WANG Liang1; WANG Wu1; HA Xiaodan3; LI Xiumei3
1.The Fifth Affiliated Hospital of Xinjiang Medical University,Xinjiang Urumqi 830000,China;2.Clinical Medicine 2020-2 of Chengdu University,Sichuan Chengdu 610106,China;3.The School of Basic Medicine of Xinjiang Medical University,Xinjiang Urumqi 830011,China.
Keywords:
esophageal cancer; exosome; cell proliferation; β-catenin; AKT
PACS:
R735.1
DOI:
10.3969/j.issn.1672-4992.2024.20.001
Abstract:
Objective:To detect the proliferation,apoptosis and migration of tumor cells and the expression of Wnt/β-catenin and PI3K-AKT signaling pathways proteins of ECA109 cells by using exosomes derived from esophageal cancer,and to explore the role of exosomes in the development of esophageal cancer cells.Methods:Exosomes were extracted from peripheral blood serum of patients with esophageal cancer by ultra-high speed centrifugation method and co-cultured with ECA109 cells to detect the changes of cell proliferation,apoptosis,migration and other biological behaviors.The expressions of AKT and β-catenin were detected by Western-blot.Results:Transmission electron microscopy,particle size measurement and marker protein results confirmed that the extracted serum exosomes from patients with esophageal cancer met the identification criteria.Compared with the blank cell group,after the intervention of exosomes,the proliferation,migration and G2+S phase of esophageal cancer cells were significantly increased,and the apoptosis rate was significantly decreased.The phosphorylation level of AKT protein in the exosome group was significantly higher than that in the blank cell group,and the expression of β-catenin was significantly lower.Conclusion:Exosomes derived from esophageal cancer can promote the activation of AKT,promote the proliferation and migration of tumor cells,and inhibit cell apoptosis,suggesting that the role of tumor-derived exosomes in tumor development should not be ignored.

References:

［1］LUO H,SUN Y,GE H,et al.Targeting endoplasmic reticulum associated degradation pathway combined with radiotherapy enhances the immunogenicity of esophageal cancer cells［J］.Cancer Biol Ther,2023,24(1):2166763.
［2］SHEN Q,HUANG Z,JIN Y,et al.Extracellular vesicles-mediated interaction within intestinal microenvironment in inflammatory bowel disease［J］.J Adv Res,2021,37:221-233.
［3］SUN H,MENG Q,CHEN R,et al.Hypoxia-inducible exosomes facilitate liver-tropic pre-metastatic niche in colorectal cancer ［J］.Hepatology,2021,74(5):2633-2651.
［4］LI B,CAO Y,FENG H,et al.Expression,regulation,and function of exosome-derived miRNAs in cancer progression and therapy［J］.FASEB J,2021,35(10):e21916.
［5］TAVASOLIAN F,MOGHADDAM AS,SAHEBKAR A,et al.Exosomes:Effectual players in rheumatoid arthritis［J］.Autoimmun Rev,2020,19(6):102511.
［6］KIMIZ-GEBOLOGLU I,ONCEL SS.Exosomes:Large-scale production,isolation,drug loading efficiency,and biodistribution and uptake［J］.J Control Release,2022,347:533-543.
［7］KRYLOVA SV,FENG D.The machinery of exosomes:Biogenesis,release,and uptake［J］.Int J Mol Sci,2023,24(2):1337.
［8］WANG C,LI Z,YUAN L,et al.Exosomes in atherosclerosis:performers,bystanders,biomarkers,and therapeutic targets ［J］.Theranostics,2021,11(8):3996-4010.
［9］FU X,SONG J,LI J,et al.The biological function of tumor-derived extracellular vesicles on metabolism［J］.Cell Commun Signal,2023,21 (1):150.
［10］VAFAEI S,ROUDI R,EBRAHIMI M,et al.Potential theranostics of circulating tumor cells and tumor-derived exosomes application in colorectal cancer［J］.Cancer Cell Int,2020,20:288.
［11］LU Tingwei,ZHANG Jianjun,CHEN Wantao.Regulation of natural killer cell activity by exosomes derived from malignant tumor cells ［J］.Journal of Shanghai Jiao Tong University (Medical Science Edition),2021,41(5):659-664.
［12］XIE P,TAN SY,ZHOU JH,et al.Transcriptome data-based status of PI3K/AKT/mTOR pathway indicates heterogeneity and immune modulation in patients with pancreatic ductal adenocarcinoma［J］.J Gene Med,2024,26(1):e3570.
［13］XU Liben,WU Chaoyang,WANG Yuandong.The role of PI3K/Akt signal pathway in the development and progression of tumor［J］.Modern Oncology,2021,29(01):177-180.
［14］YU L,WEI J,LIU P.Attacking the PI3K/Akt/mTOR signaling pathway for targeted therapeutic treatment in human cancer［J］.Semin Cancer Biol,2022,85:69-94.
［15］ALBRECHT LV,TEJEDA-MUNOZ N,DE ROBERTIS EM.Cell biology of canonical Wnt signaling［J］.Annu Rev Cell Dev Biol,2021,37:369-389.
［16］YU F,YU C,YE L,et al.Wnt/β-catenin signaling in cancers and targeted therapies［J］.Signal Transduct Target Ther,2021,6(1):307.

Memo

Memo:
新疆维吾尔自治区卫生计生委青年医学科技人才专项（编号：WJWY-202146）
Last Update: 1900-01-01