|Table of Contents|

Impacts of kaempferol on proliferation,apoptosis and epithelial-mesenchymal transition of nasopharyngeal carcinoma cells by regulating the AKT/GSK-3β/Snail signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 19
Page:
3677-3683
Research Field:
Publishing date:

Info

Title:
Impacts of kaempferol on proliferation,apoptosis and epithelial-mesenchymal transition of nasopharyngeal carcinoma cells by regulating the AKT/GSK-3β/Snail signaling pathway
Author(s):
MING Hua1; HUANG Xiaoou2; LIN Wuhua1; GUO Yannan3
1.Department of Oncology,Sichuan Armed Police Corps Hospital,Sichuan Chengdu 610059,China;2.Department of Hematology,West China Hospital,Sichuan University,Sichuan Chengdu 610041,China;3.Renal Immunization in West China Second Hospital of Sichuan University,Sichuan Chengdu 610041,China.
Keywords:
kaempferol; nasopharyngeal cancer; proliferation; apoptosis; epithelial-mesenchymal transformation; AKT/GSK-3β/Snail signaling pathway
PACS:
R739.62
DOI:
10.3969/j.issn.1672-4992.2024.19.009
Abstract:
Objective:To investigate the impacts of kaempferol on proliferation,apoptosis,and epithelial-mesenchymal transition(EMT) of nasopharyngeal carcinoma cells,and to explore its mechanism of action.Methods:CNE-2 cells were grouped into a control group,a low concentration kaempferol group,a medium concentration kaempferol group,a high concentration kaempferol group,and a high concentration kaempferol+SC-79(AKT activator) group.CCK-8 method and cloning experiment were applied to measure cell proliferation,flow cytometry was applied to detect cell apoptosis,Weatern blot detected the expression level of B cell lymphoblastoma 2(Bcl-2),Bcl-2 associated X protein(Bax),aspartate proteolytic enzyme 3(Caspase-3),Vimentin,epithelial cadherin(E-cadherin),neural cadherin(N-cadherin),protein kinase B(AKT),phosphorylated Akt(p-AKT),glycogen synthase kinase-3β(GSK-3β),phosphorylated GSK-3β(p-GSK-3β),and Snail.A nude mouse model of nasopharyngeal carcinoma was constructed and grouped into NC group,kaempferol group,and kaempferol+SC-79 group.The tumor mass and volume were measured,and the expression of p-AKT,p-GSK-3β,and Snail protein in the transplanted tumor tissue was detected using immunohistochemistry.Results:Kaempferol had no significant effect on the viability of human nasopharyngeal carcinoma epithelial cells(P＞0.05).The vitality of nasopharyngeal carcinoma cells gradually decreased with the increase of kaempferol concentration(P＜0.05).CNE-2 was selected as the subsequent experimental cell,and 25,50 and 100 μmol/L kaempferol were selected as the subsequent experimental concentrations.Compared with the control group,the cell viability,Bcl-2,N-cadherin,Vimentin,p-AKT/AKT,p-GSK-3β/GSK-3β,and Snail levels in the low,medium,and high kaempferol groups obviously decreased,and the apoptosis rate,the level of Bax,Caspase-3,and E-cadherin increased(P＜0.05).Compared with the high concentration kaempferol group,the cell viability,Bcl-2,N-cadherin,Vimentin,p-AKT/AKT,p-GSK-3β/GSK-3β,and Snail levels in the high concentration kaempferol+SC-79 group obviously increased,and the apoptosis rate,the level of Bax,Caspase-3,and E-cadherin decreased(P＜0.05).Kaempferol was able to inhibit the mass and volume of transplanted tumors,reduce the expression of p-AKT,p-GSK-3β,and Snail proteins(P＜0.05).SC-79 reversed the inhibitory effect of kaempferol on transplanted tumors and promoted the expression of AKT/GSK-3β/Snail pathway proteins(P＜0.05).Conclusion:Kaempferol can inhibit the proliferation and EMT of nasopharyngeal carcinoma cells,promote cell apoptosis,and its mechanism of action may be related to the inhibition of AKT/GSK-3β/Snail signaling pathway.

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Last Update: 2024-08-30