|Table of Contents|

The expression levels and biological function of LRP1 in lung cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 19
Page:
3643-3647
Research Field:
Publishing date:

Info

Title:
The expression levels and biological function of LRP1 in lung cancer cells
Author(s):
WANG Haiqiang; ZHANG Tianyi; ZHANG Weifeng; YIN Xunliang; ZHOU Yong'an; ZHAO Zhengwei
Department of Thoracic Surgery,the Second Affiliated Hospital of Air Force Medical University,Shaanxi Xi'an 710038,China.
Keywords:
lung cancer; A549 cells; LRP1; proliferation; migration; invasion
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2024.19.003
Abstract:
Objective:To explore the expression levels and biological significance of low density lipoprotein receptor-related protein 1(LRP1) in lung cancer cells.Methods:Conventional culture of human lung cancer A549 cells,and RT-PCR method to detect the mRNA level of LRP1 in A549 cells.To construct siRNA infected cells targeting LRP1,using green fluorescent labeling vectors,observe infection efficiency through fluorescence microscopy,detect knockdown efficiency using RT-PCR,and detect changes in LRP1 expression levels using Western blot.A549/LRP1 siRNA cell lines were selected for functional experiments,and CCK-8 assay was used to detect changes in cell proliferation ability,scratch assay was used to detect changes in cell migration ability,and Transwell invasion chamber assay was used to detect changes in cell invasion ability.Results:From the quantitative PCR results,it can be seen that in A549 cells,compared to the NC group,the LRP1 gene knockout efficiency in the KD group was 77.7%.The mRNA level of LRP1 in the KD group cells was significantly lower than that in the NC group,and the difference was statistically significant(P＜0.05).Western blot analysis showed that the expression level of LRP1 in the KD group was significantly downregulated compared to the NC group.The CCK-8 detection results showed that compared to the NC group,the KD group had a significant decrease in cell proliferation multiple on Day 5,and the difference was statistically significant(P＜0.05).The scratch experiment results showed that compared with the NC group,the KD group showed a significant decrease in cell migration rates after 8 and 24 hours of scratch,with statistical significance(P＜0.05).The results of Transwell invasion experiment showed that the invasion and metastasis rate of KD group cells significantly decreased after 24 hours of incubation in the invasion chamber,and the difference was statistically significant(P＜0.05).Conclusion:LRP1 plays an important role in the biological function of lung cancer A549 cells.Downregulation of LRP1 can inhibit the proliferation,migration,and invasion ability of A549 cells.The research on the upstream and downstream signaling pathways of LRP1 molecule has certain clinical reference value in the diagnosis and treatment of lung cancer.

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Memo

Memo:
National Natural Science Foundation of China（No.81402411）;国家自然科学基金资助项目（编号：81402411）
Last Update: 2024-08-30