|Table of Contents|

Silencing PHLDA2 to inhibit EMT and autophagy in pancreatic cancer through PI3K/AKT/mTOR pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 18
Page:
3447-3453
Research Field:
Publishing date:

Info

Title:
Silencing PHLDA2 to inhibit EMT and autophagy in pancreatic cancer through PI3K/AKT/mTOR pathway
Author(s):
Amutijiang·Mahemuti1; Mairepati·Aierkaixi2; ZHENG Jianjiang2; Dilixiati·Alimu2; CHEN Xiong1
1.Pancreatic Department,Hepatobiliary and Pancreatic Diagnosis and Treatment Center of Xinjiang Uygur Autonomous Region People’s Hospital,Xinjiang Urumqi 830001,China;2.Imaging Department,the Eighth Affiliated Hospital of Xinjiang Medical University,Xinjiang Urumqi 830001,China.
Keywords:
pancreatic cancer; phosphatidylinositol kinase; epithelial mesenchymal transition; autophagy; proliferation; apoptosis
PACS:
R735.9
DOI:
10.3969/j.issn.1672-4992.2024.18.008
Abstract:
Objective:To investigate the molecular mechanism of silencing human plec substrate protein homeodomain family A member 2 (PHLDA2) gene through phosphatidylinositol kinase (PI3K)/silk threonine protein kinase (AKT)/rapamycin target protein (mTOR) signaling pathway to inhibit epithelial mesenchymal transition (EMT) and autophagy in pancreatic cancer cells.Methods:Firstly,the expression of PHLDA2 mRNA in human pancreatic cancer cells PANC-1,SW1990 and normal pancreatic duct epithelial cells HPDE6-C7 was detected by qRT-PCR.Then,PANC-1 cells were divided into control group,PHLDA2 silencing group,and negative group.After 48 hours of cultivation,cell proliferation was detected with MTT method,cell apoptosis was detected with flow cytometry,and Western blot was used to detect EMT markers including Vimentin,N-cadherin,and E-cadherin,autophagy markers including Beclin1 and microtubule associated protein light chain 3 (LC3),as well as p-PI3K,p-AKT,and p-mTOR proteins.Results:Compared with HPDE6-C7,the expression level of PHLDA2 mRNA in PANC-1 and SW1990 was significantly higher,and PANC-1 was higher than SW1990 (P＜0.05).Compared with the control group,PHLDA2 mRNA expression level in PHLDA2 silencing group was significantly less (P＜0.05).Compared with the control group,cell proliferation rate in silencing group was significantly lower,apoptosis rate was higher,E-cadherin was more,the expressions of Vimentin,N-cadherin,Beclin1,LC3,p-PI3K,p-AKT,and p-mTOR were less,too(P＜0.05).Conclusion:Up-regulation of PHLDA2 gene may be involved in the occurrence of pancreatic cancer,which may be involved in the occurrence of tumor EMT and autophagy by activating PI3K/AKT/mTOR signaling pathway.PHLDA2 may be a new site for targeted intervention in pancreatic cancer.

References:

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新疆少数民族科技人才特殊培养计划科研项目（编号：2021D03019）
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