|Table of Contents|

The mechanistic analysis of Siha apoptosis and ferroptosis caused in cervical cancer cells by Pseudolaric acid B up-regulation of HMOX1

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 18
Page:
3413-3419
Research Field:
Publishing date:

Info

Title:
The mechanistic analysis of Siha apoptosis and ferroptosis caused in cervical cancer cells by Pseudolaric acid B up-regulation of HMOX1
Author(s):
DONG Jingjing1; SHI Shaoqing2; LI Yuqiong1; NIU Lulu1; SUN Ruifen1
1.Yunnan University of Traditional Chinese Medicine,Yunnan Kunming 650500,China;2.The First Affiliated Hospital of Kunming Medical University,Yunnan Kunming 650032,China.
Keywords:
Pseudolaric acid B (PAB); ferroptosis; cervical cancer
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2024.18.003
Abstract:
Objective:To explore the mechanism of apoptosis and ferroptosis mediated by PAB（Pseudolaric acid B，PAB）in cervical cancer Siha cells.Methods:The cytotoxicity of PAB on Siha was determined using the CCK8 test.Annexin V-FITC and PI double fluorescence labeling were used to detect apoptosis.Fluorescence microscopy was used to assess nuclear morphology and cell death after labeling with PI and Hochest.The cell cycle and intracellular reactive oxygen species (ROS) levels were determined using flow cytometry.Inverted fluorescence microscopy with BODIPYTM 581/591 C11 labeling to detect intracellular lipid peroxidation products.Fluorescence microscopy was used to monitor intracellular mitochondrial membrane potential changes using JC-1 labeling.Western blot analysis revealed the expression of ferroptosis-associated proteins GPX4,SLC7A11,Ferritin,and HMOX1,as well as apoptosis-related proteins PARP,c-IAP1,c-IAP2,XIAP,and Survivin.The mRNA expression levels of ferroptosis-related genes were determined using RT-PCR.Results:PAB dramatically reduced the proliferation of cervical cancer Siha cells,induced apoptosis,and stopped the cell cycle.Second,PAB increased intracellular ROS levels,lipid peroxide buildup,and mitochondrial membrane potential increases.Meanwhile,it increased the expression of HMOX1 protein and mRNA,decreased the expression of GPX4,SLC7A11 protein and mRNA,and decreased the expression of Ferritin,c-IAP1,c-IAP2,XIAP,and Survivin.Conclusion:PAB dramatically increased cell death in cervical cancer Siha cells,and the mechanism of action may be connected to HMOX1 overexpression to trigger apoptosis and ferroptosis.

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云南省科技厅科技计划项目［编号：202201AT070043，2019FF002(-011)］
Last Update: 1900-01-01