|Table of Contents|

Nonylphenol promotes invasion and migration of colorectal cancer cells by altering tumor microenvironment through NF-κB signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 18
Page:
3406-3412
Research Field:
Publishing date:

Info

Title:
Nonylphenol promotes invasion and migration of colorectal cancer cells by altering tumor microenvironment through NF-κB signaling pathway
Author(s):
ZHANG Nianjie; ZHANG Yuanwei; YIN Shuo; HE Nian; DENG Xun; QU Yan; WANG Biao; YANG Xuefeng
The Second Affiliated Hospital of Zunyi Medical University,Guizhou Zunyi 563000,China.
Keywords:
Nonylphenol; colorectal cancer; NF-κB signaling pathway; tumor microenvironment; invasion and migration
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2024.18.002
Abstract:
Objective:To investigate the effect of Nonylphenol (NP) on invasion and migration of HCT-116 cell tumor microenvironment and its relationship with NF-κB signaling pathway.Methods:After different concentrations of NP,Transwell assay was used to detect the changes of invasion and migration ability of HCT-116 cells.The contents of pro-inflammatory factor IL-6 and TNF-α in the supernatant of HCT-116 cells were detected by ELISA.The expression of NF-κB signaling pathway was detected by qRT-PCR and Western blot.The subcellular localization of p-p65 was detected by immunofluorescence technique.Results:Transwell analysis showed that NP intervention significantly promoted the invasion and migration ability of HCT-116 cells compared with Control group (P＜0.01).Compared with NP (10-6 mol/L) group,the invasion and migration ability of colorectal cancer cells were significantly inhibited after inhibition of NF-κB pathway (P＜0.01).ELISA analysis showed that NP intervention significantly promoted the production of pro-inflammatory cytokines IL-6 and TNF-α compared with Control group (P＜0.01).Compared with NP (10-6 mol/L) group,the contents of IL-6 and TNF-α were significantly decreased after inhibition of NF-κB pathway (P＜0.05).qRT-PCR and Western blot analysis showed that NF-κB signaling pathway was significantly activated in NP intervention groups compared with Control group (P＜0.05 or P＜0.01).Compared with NP (10-6 mol/L) group,the expression of NF-κB signaling pathway in NP (10-6 mol/L) + (-)-DHMEQ group was significantly inhibited (P＜0.01).Immunofluorescence analysis showed that compared with Control group,the expression of p-p65 in the nucleus of cells in NP intervention group was significantly increased (P＜0.01).Compared with NP (10-6 mol/L) group,the expression of p-p65 in the nucleus of NP (10-6 mol/L) + (-)-DHMEQ group was significantly inhibited (P＜0.01).Conclusion:NP changes the tumor microenvironment by activating NF-κB signaling pathway and promotes the invasion and migration of colorectal cancer cells.

References:

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Memo

Memo:
贵州省科技计划项目(编号：黔科合-ZK［2022］一般631);贵州省卫生健康委科技基金(编号：gzwjkj2020-1-099);遵义医科大学第二附属医院硕士启动基金(编号：SQ-2021-14，SQ-2021-15)
Last Update: 1900-01-01