|Table of Contents|

Expression of O-GlcNAc transferase in nasopharyngeal carcinoma tissues and cells and its influence on malignant progression of tumor

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 17
Page:
3234-3240
Research Field:
Publishing date:

Info

Title:
Expression of O-GlcNAc transferase in nasopharyngeal carcinoma tissues and cells and its influence on malignant progression of tumor
Author(s):
REN Wenjing1; GUI Haifeng1; QIAO Ping1; ZHU Yingchun1; XU Linli1; YANG Bin2; ZHANG Haiyan1
1.Cangzhou Medical College,Hebei Cangzhou 061000,China;2.Cangzhou People's Hospital,Hebei Cangzhou 061000,China.
Keywords:
nasopharyngeal carcinoma; O-GlcNAc transferase; O-GlcNAcylation; proliferation; apoptosis
PACS:
R739.6
DOI:
10.3969/j.issn.1672-4992.2024.17.011
Abstract:
Objective:To explore the effects of O-linked β-N-acetylglucosamine（O-GlcNAc) transferase（OGT) on cell proliferation,apoptosis,migration,and invasion of nasopharyngeal carcinoma（NPC).Methods:The expression of OGT in NPC tissues and cells was deteced using quantitative real-time polymerase chain reaction（qRT-PCR).The proliferation,apoptosis,migration,and invasion of NPC cells were detected using CCK-8,colony formation,flow cytometry,scratch wound healing and Transwell assay,respectively.The expression of OGT protein were detected using immunohistochemistry（IHC) and Western blot.Results:The expression of OGT was upregulated in NPC tissues and cells（P＜0.05),and high expression of OGT was significantly correlated with T stage（P=0.038),lymph node metastasis（P=0.028) and clinical stage（P=0.000) of NPC patients,which was associated with poor prognosis（P＜0.05).Compared with the sh-NC group,OGT knockdown suppressed the proliferation of NPC cells in vitro and the growth of xenograft tumors in vivo,induced apoptosis,and inhibited cell migration and invasiveness（P＜0.05).Conclusion:OGT was highly expressed in NPC tissues and cells,and was positively correlated with tumor progression and poor prognosis.OGT may be a potential new prognostic biomarker and therapeutic target for NPC patients.

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Memo

Memo:
河北省卫生健康委科研基金项目（编号：20222135）
Last Update: 2024-07-31