|Table of Contents|

Synergistic effect of fecal microbiota transplantation on fluorouracil against colon tumor in mice

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 17
Page:
3213-3219
Research Field:
Publishing date:

Info

Title:
Synergistic effect of fecal microbiota transplantation on fluorouracil against colon tumor in mice
Author(s):
YANG Qiuyu1; SUN Yuhang2; TAN Mengtian1; BAI Jing2; LI Lei2; YANG Yong2; WU Fang2; LI He1; FU Zexian1
1.Hebei University of Engineering,Hebei Handan 056000,China;2.Affiliated Hospital of Hebei University of Engineering,Hebei Handan 056038,China.
Keywords:
fecal microbiota transplantation; tumor immunization; tumor-bearing mice; subcutaneous tumor model
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2024.17.008
Abstract:
Objective:To observe the synergistic effect of fecal microbiota transplantation on the inhibition of graft tumor growth by fluorouracil in MC38 mouse colon cancer cells loaded mice.Methods:21 female C57BL/6N mice were selected as study subjects and divided into blank group,control group and experimental group.MC38 mouse colon cancer cells were used to inoculate the mice subcutaneously on the back of the right forelimb to construct a colon cancer hormonal tumor model.The blank group was treated without any treatment,and its feces were taken to make the intervention bacterial solution,and the experimental and control groups were given 0.1 mL bacterial solution/saline gavage once every two days since the grouping until the end of the experiment.On the 6th day after tumor formation,fluorouracil was injected intraperitoneally for 5 consecutive days at a dose of 65 mg/kg,and the experimental animals were executed after 3 days to calculate the tumor volume and tumor suppression rate.The colorectal tissues were observed by HE staining.Serum levels of interleukin-6（IL-6) and tumor necrosis factor alpha（TNF-α) were detected by enzyme-linked immunosorbent assay（ELISA),and intestinal flora was detected and analyzed by 16S sequencing.Results:Compared with the blank group,the body weight of the model mice in both the experimental and control groups was significantly reduced（P＜0.05),but no significant difference in body weight change was observed between the control and experimental groups（P＞0.05).The average volume of the control group's loaded tumors was larger than the average volume of the experimental group's loaded tumors（P＜0.05).Compared with the control group,more crypt cells with more intact villi were seen microscopically in the histopathologic histological sections of colorectal tissue in the experimental group.The serum level of IL-6 in the experimental group was higher than that in the control group and the blank group(P＜0.05).The serum level of TNF-α in the experimental group and the control group was significantly higher compared with that in the blank group（P＜0.05),but the difference between the experimental group and the control group was not statistically significant（P＜0.05).The 16S sequencing results suggested that,after the intervention of autologous bacterial transplantation,the intestinal bacterial flora of the experimental group was closer to that of the blank group,and the bacterial abundance in the control group was the lowest,but no significant difference was seen among the three groups（P＜0.05).Further analysis of the differences in the flora of the three groups revealed that at the genus level,Mar-Odoribacter and Tan-Parabacteroide in the experimental group and Ent-Escherichia-Shigella in the control group were more enriched compared with the blank group.Conclusion:Up-regulation of Mar-Odoribacter and Tan-Parabacteroide bacteria and down-regulation of Ent-Escherichia-Shigella bacteria after fecal microbiota transplantation protects the intestinal mucosa,improves the metabolism of the organism,activates the immune system of the organism,and promotes the expression of IL-6 and TNF-α cytokines,which enhances the anti-tumor immune ability,and has an effect on the fluorouracil synergistically inhibits the proliferation of transplanted tumors in MC38 hormonal mice.

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Memo:
河北省教育厅科学研究项目（编号：ZD2022039）
Last Update: 2024-07-31